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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.4

The Analysis on Isolation Method of Cervus elaphus yarkandensis Preantral Follicles
Author of the article:WANG Jian-hong1, CHEN Jing-bo2 , HAILI Qie-mu2
Author's Workplace:(1. School of Life Sciencs, Xinjiang Normal University, Urumuqi 830054, China;2. Xinjiang Institute of Animal Science, Urumuqi 830000, China)
Key Words:Cervus elaphus yarkandensis; preantral follicles; isolation method
Abstract:To identify an high-efficient method for isolation of Cervus elaphus yarkandensis preantral follicles, this paper compared the isolation effects of the comb-scrape method, the enzymatic digestion method, and the cutting method by observing and recording the number of preantral follicles, the rate of normal follicles and their livability cultured for 72 hours in vitro. The results showed that the enzyme digestion method harvested significantly more preantral follicles than that of the comb-scrape and the cutting methods. However, the rate of normal follicles isolated by the comb-scrape method and cutting method was significantly higher than that of the enzymatic method. In addition, the livability of preantral follicles cultured for 24, 48, or 72 hours isolated using the comb-scrape method and the cutting method was significantly higher than that of the enzyme digestion method (2448 hours after culture in vitro P <0.01, 72 hours after culture in vitro P <0.05). The comb-scrape method is an high-efficient method for isolating preantral follicles in Cervus elaphus yarkandensis ovary.
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