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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.3

Improving the Methods for Primary rat Schwann Cell Culture
Author of the article:CHEN Xiang, ZHAO Ming, ZHOU Hua-shan, HU Huo-zhen*
Author's Workplace:(College of Life Sciences, Sichuan University, Chengdu 610064, China)
Key Words:Schwann cell; primary culture
Abstract:To get more purified Schwann cells, we improved the common cytarabine arabinoside treatment and differential adhesion methods for primary rat Schwann cell culture. First, we used the cytosine arabinoside (Ara-c) to kill the most of fibroblasts. Second, we treated with anti-Thy-1.1 antibody and rabbit complement to remove residual fibroblasts. In addition, to avoid a large number of Schwann cells death caused by bad state of Schwann cells, we improved the concentration of anti-Thy-1.1 antibody and rabbit complement, processing duration and so on. This method could make the purity of Schwann cells increased from 90% to 99%.
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