A PCR-DGGE-based Approach for Identification of F1 Hybrids of Rock Carp (Procypris rabaudi) × Fancy Carp (Cyprinus carpio)
Author of the article:GUO Rui1#, YANG Shi-yong1,2#, LIU Cong 3, ZHANG Xiu-yue 1, SONG Zhao-bin 1*
Author's Workplace：(1. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610065, China; 2. Department of Aquaculture, Sichuan Agricultural University, Ya’an, Sichuan Province 625014, China; 3. Tongwei Company Ltd, Chengdu 610041, China)
Key Words：hybrid; allele; PCR-DGGE; IRBP
Abstract： An approach based on denaturing gradient gel electrophoresis (DGGE), and a nuclear marker, interphotoreceptor retinoid-binding protein (IRBP) gene, were introduced in the field of identification of interspecific fish hybrids. Using this method, the F1 hybrids produced by Procypris rabaudi × Cyprinus carpio were successfully identified. The alleles of IRBP gene exon 1 were clearly separated for P. rabaudi, C. carpio, and their F1 hybrids using DGGE, as well as the ones of F1 hybrids which were from either of the parents. The sequence identity of alleles in rock carp was 99.8%, and which was 98.8% in fancy carp. However, the identity of alleles in F1 hybrids was 95.8%~96.7%, which was almost alike that between rock carp and fancy carp (95.5%~96.9%). This approach would benefit the hybrids investigation in fish in the future.