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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.2

Isolation and Identification of the Pathogenic Shigella of Rhesus Macaque and Its Pharmacosensitive Test
Author of the article:CHEN Xi-wen1,2, GUO Xiao-ping2, WANG Xiong-qing1,2*, DENG Yan1, PU Zhong-hui1, MA Ying 2
Author's Workplace:(1. Institute of Applied Animal Technology, Mianyang Normal University, Mianyang, Sichuan Province 621000, China; 2. Key Laboratory for Molecular Biology and Biopharmaceuticals, Mianyang Normal University, Mianyang, Sichuan Province 621000, China)
Key Words: Macaca; Shigella; isolation and identification; pharmacosensitive test
Abstract:Objective  To investigate the pathogenic Shigella of rhesus macaque and find effective treatment measures(drug). Method  Varieies of culture mediums were adopted for the 13 samples, and the Gram method staining was used for preliminary classification, routine physiology and biochemistry assays were also operated. Mice were used for the pathogenic assays. The drug susceptibility of all the pathogen against 23 antibiotics was tested. Results  12 strains of Shigella: Shigella dysenteriae (A group) one stubs, Shigella flexneri (B group) ten stub, Shigella sonnei (D group) one stub, were isolated from feces of thirteen samples (92.31%). The antibiotic sensitivity test showed that cefotaxime produced the highest sensitivity (86.49%), followed by cefatriaxon (75.00%) and ceftazidime (66.67%). Conclusion  These results preliminary indicate that the rhesus macaque infected with Shigella is prevalent, and the most effectively drugs for Shigella is cefotaxime.
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