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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2005 Vol.24 No.1

Advances in Mammalian Sperm Separation Methods
Author of the article:WEI Kun, PAN Juan
Author's Workplace:(Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064)
Key Words:sex control; sperm separation; method for assessing the purity of separated sperm
Abstract:The effective approach for achieving sex control in mammals at the present time is separating X- from Y-bearing sperm followed by artificial insemination (AI) or in vitro fertilization (IVF). Many techniques have been used to separate mammalian X- and Y- bearing sperm through past decades, mainly including method of various gradients, swim up, Sephadex columns and H- Y antigen, all of which focus on physical or immunological separation. But those procedures have not been scientifically proved as effective by resulting in unconfirmed separation followed by inconsistent birth ratio results. At present, flow cytometric sperm sorting based on X and Y sperm DNA difference has been confirmed as the only effective method for sexing the spermatozoa of mammals after DNA analysis by fluorescent in situ hybridization. This paper reviews the history and current status of mammalian sperm separation and the method for assessing the purity of separated sperm.
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