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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2005 Vol.24 No.1

Establishment of Severe Traumatic Brain Closed Injury Animal Model in Non-anesthetic Mice
Author of the article:QU Qiang1,2, SHI Zhong2, SU Yong-ping*1, YIN Zhi-yong3, XIAO Tao-yuan4, AI Guo-ping1, LU Jian-hua1
Author's Workplace:(1. Institute of Combined Injure of PLA, the Third Military University, Chongqing 400038; 2. Emergency Department of Xinqiao Hospital, the Third Military University; 3. Institute of Field Surgery, the Third Military University; 4. Central Laboratory, the Third Military University)
Key Words:traumatic brain closed injury (closed TBI); animal model; mouse
Abstract:Objective To establish an animal model of severe traumatic brain closed injury (closed TBI) in traffic or war in mice. Methods Severe closed TBI in mice was performed by a BIM-Ⅲ biomechanical machine with non-anesthesia. At the time points of 2h, 8h, 24h, 72h, 120h, 1w, 2w after injury, the neurobehavior of mice was measured by neurological examination and motor functional test. Besides, cerebral and lung fluid content, mortality rate, pathologic and ultrastructure picture were analyzed. Results 48h after TBI, the neurobehavioral and motor function scores decreased significantly than that in control group (P<0.05), the mortality rate was high, there were no significant differences in lung fluid content among dead mice in different groups, but the fluid content in injury group was significantly higher than that in control group (P<0.05), and brain injury such as cells tumefaction, necrosis were confirmed by pathologic and ultrastructure technique. Conclusion The imitation would be a valuable animal model for experimental investigation of severe TBI in accident of traffic or war.
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