Cloning and Sequence Analysis of Hydrogenosomal Adenylate Kinase (AK) of Trichomonas vaginalis
Author of the article:TIE Chao-nan1, WANG Ya-jing*1, XIE Hui1, BI Shi-liang2, LIU Pei-na1, LIAO Lin1
Author's Workplace：（1. Department of Parasitology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041; 2. Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University）
Key Words：Trichomonas vaginalis; hydrogenosomal; adenylate kinase (AK); clone; sequence analysis
Abstract：Objective To clone the gene of AK and analyse encoding sequence of AK gene of Trichomonas vaginalis. Methods A pair of primers was designed according to the known sequence of AK gene. The AK gene fragment was amplified by PCR, and ligated to a sequencing, pMD18-T simple vector. Positive recombinants were detected by PCR, digested by restriction enzyme and sequenced with dideoxy nucleotide chain termination method. Result The sequence analysis showed that the sequenced protein showed 99.9% of homology with the AK amino acid sequence in Genbank. Conclusion The gene of AK has high homology with the AK gene sequence in Genbank.