Isolation and Identification on Pathogens of Musk Deer Abscess Disease and Antibiotic Susceptibility Assay
Author of the article:ZHAO Ke-lei1, LI Xu-xin2, PALAHATI Paha'erding1, ZENG Bo3, ZHANG Xiu-yue1, YUE Bi-song1*
Author's Workplace： (1.Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Miyaluo Farm of Forest Musk Deer, Lixian, Sichuan Province 623102, China; 3. Key Laboratory of Bioresources and Ecoenvironment(Ministry of Education), Sichuan University, Chengdu 610064, China)
Key Words：musk deer; pathogens; isolation; identification; drug susceptibility
Abstract：Objective The most common and important factors which restrict the development of musk deer industry was abscess disease and it was a great threat. This experiment was aimed to find the main pathogenic bacteria and effective treatment measures (drug). Method Kinds of culture mediums were adopted for the 28 samples, and the Gram method staining was used for preliminary classification, then the 16S rRNA sequencing of all the pathogenic bacteria was examined, routine physiology and biochemistry assays were operated though BIOLOG Microlog System Release 4.2. KM mice were used for the pathogenic assays. The drug susceptibility of all the pathogen against 22 antibiotics was tested. Results Incubation of the abscess samples showed that the isolate rate of Arcanobacterium pyogenes was 100%, Pseudomonas aeruginosa was 21.4%, Bacillus cereus was 14.3%. 16S rRNA sequencing and pathogenic assays finally confirmed that the main pathogenic bacteria was A. pyogenes. Conclusion These results preliminary indicate that the musk deer abscess disease was firstly caused by A. pyogenes, then co-infected with other bacteria; The most effectively drugs for A. pyogenes is ciprofloxacin and simultaneously for these three pathogens are ciprofloxacin and clindamycin.