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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2011 Vol.30 No.4

Comparative Studies on the Immune Responses of Tibet and Landrace Piglets to FMD Vaccine
Author of the article:HE Han-bing1#, YIN Luo-rong3#, YANG Xiao1#, LI Jiang-ling2, BAI Guang-ming1, LIU Rui2, ZENG Kai2, Lü
Author's Workplace:(1. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Sichuan Animal Science Academy, Chengdu 610035, China; 3. Chengdu Vocational College of Agricultural Science and Technology, Chengdu 611130, China)
Key Words:Tibetan pig; Landrace pig; immune-related genes; expression; FMD immunity
Abstract:Objective To compare of the changes in the immune system of Tibetan pigs and Landrace after inoculation of inactivated Foot-and-Mouth Disease (FMD) vaccine, and analyze the differences in immune response, and lay the foundation for investigating the genetic characteristics and resistance of Tibetan pigs. Methods 8-day-old Tibet and Landrace pigletswere intramuscularly immunized withthe inactivated FMD vaccine, and their blood were collected before and after vaccination on 1, 2, 4 and 6 weeks respectively to test the changes of immune cells, specific antibody and the expression level of immune genes by ELISA and quantitative RT-PCR. Results It was found that the white blood cells and anti-FMD virus antibody content of Tibet piglets significantly increased from 4 to 6 weeks post immunization in comparison with those of Landrace piglets (P<0.05); The expression level of TLR7 and CD4 genes of Tibet piglets were markedly higher than Landrace (P<0.05). In contrast, the expression of TLR4, TLR9 and IL10 in Landrace piglets rose significantly than Tibet piglets from 2 to 6 weeks post immunization (P<0.05). Conclusion The hum oral immunity of Tibetan pig to FMD were significantly enhanced compared with that of Landrace pig , which is correlated with the increased expression of CD4 and TRL7 genes of immune cells; Otherwise, the expression level of IL-10, TLR4 and TLR9 genes mounted remarkably in immune cells of Landrace pig in comparison with Tibetan pig.
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