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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2011 Vol.30 No.4

Polymorphisms of Milk Epithelial MUC1 Gene and Protein of Goat
Author of the article:HUANG Lin1, WANG Yong2, KUI Xiao1, YANG Fa-long1, XU Ya-ou1, ZHENG Yu-cai1*
Author's Workplace:(1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China; 2. The Qinghai-Tibet Plateau Animal Genetic Resources Protection and Utilization of Sichuan Province Key Laboratory, Southwest University for Nationalities, Chengdu 610041, China)
Key Words:goat; milk; MUC1; genetic polymorphism
Abstract:The objective of the study was to examine the genetic polymorphisms of milk MUC1 gene of goat breeds in Sichuan province. Using primers designed according to partial sequences of goat MUC1 gene from GenBank, a PCR method was established to amplify the variable number tandem repeats of goat MUC1 gene. The amplified fragment was 1.7~2.2 kb in length. A total of 3 alleles and 5 genotypes were detected in the four goat breeds studied, with high proportion of homozygote, and there were differences in genotype and allele frequencies among the goat breeds. Our experiment has provided a reliable protocol for assaying genetic polymorphisms of goat milk MUC1.
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