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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2011 Vol.30 No.4

Effects of Cu2+ to Sperm Motility of the Loach
Author of the article:CHEN Yu-ming, HU Jia-hui*, CHEN Jie, WANG Yue-wen
Author's Workplace:(Department of Life Sciences, Liaocheng University, Liaocheng, Shandong Province 252059, China)
Key Words:Cu2+; loach; sperm motility; toxicity
Abstract:To understand the sensitivity of sperm from freshwater fishes to Cu2+ on different moving conditions, the motility of the moving sperm and the resting sperm of the loach Misgurnus anguillicaudatus were examined by a computer-assisted semen analyzer (CASA). The percentage, the duration and the relative velocity of sperm movement were determined at post-activation respectively, after the sperm had been incubated in solutions containing Cu2+ (10 mM, 30 mM, 60 mM and 100 mM) for 0 h or 2 h, basing on the preliminary experiment. The results showed that the motility of both the moving sperm and the resting sperm were reduced significantly. However, the motility of the moving sperm was much more strongly affected at low concentrations of Cu2+ (10 mM, 30 mM). So did the motility of the resting sperm at high concentrations(60 mM, 100 mM). These results suggested that the loach sperm was sensitive to Cu2+, and the sensitivity of the moving sperm was different from that of the resting sperm. And Cu2+ was harmful for the loach sperm, which might adverse to reproduction of freshwater fishes.
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