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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2010 Vol.29 No.6

Experimental Research of Myocardial Ischemia-reperfusion Injury Model on Rats
Author of the article:LU Ren-fu, JA Ke, WU Qing-chen*
Author's Workplace:(Department of Cardiothoracic Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China)
Key Words: myocardial ischemia-reperfusion; cellulate ultrastructure; rat; model
Abstract:Objective To prepare a new type of acute myocardial ischemia-reperfusion injury model by a method meeting the needs of clinical practice. Methods 20 female SD (Sprague-Dawley) rats were randomly divided into two groups: (1) control group and (2) experimental group. The method, in which myocardial ischemia was caused by the ligation of the aortic root for 5 min and then reperfusion was conducted for 30 min, was used to establish an acute myocardial ischemia-reperfusion model. Changes in myocardial ultrastructure were studied through transmission electron microscopy. Furthermore, the contents of maleic dialdehyde (MDA) and the activities of superoxide dismutase (SOD) from the myocardial tissue were determined. Results The myocardial ultrastructure indicated that there were more serious damages in myocardial tissue and mitochondria from the experimental group than the control group. The contents of myocardial MDA in the experimental group was significantly higher (P<0.01) than the control group, whereas the activities of SOD in the experimental group was significantly lower than the control group (P<0.01). Conclusion We developed a new, simple, operable method for establishing myocardial ischemia-reperfusion injury model, which could provide lots of available materials for further research.
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