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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2009 Vol.28 No.6

Advances of the Research on Isolation and Influencing Factors of the Domestic Preantral Follicles in vitro
Author of the article:ZHANG Yao-jun, WANG Zhao-hui, WEN Rui-li, SHI Hong, WU Ming-yao*
Author's Workplace:(Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China)
Key Words:domestic animals; preantral follicles; isolation in vitro; influencing factors
Abstract:
In the mammalian ovary, about 99% of the follicles present in the cortex are in the form of a non-cavity. It is essential for isolating preantral follicles effectively. The separation of follicles is established in rodents. However, this remains a challenge in livestock which has constrained research. The separation methods for livestock animals preantral follicles can be largely divided into three categories: enzymatic digestion method, mechanical separation, combined enzyme-mechanical separation. According to the different tools researchers use, mechanical separation is generally divided into the following categories: cutting method, comb-scrape method, micromanipulation, and grinding. This article reviews the methodological history of isolating preantral follicles in vitro, focuses on the method for isolation of preantral follicles in vitro and influencing factors, such as animal species, age, processing time, and ovarian size and development, thus laying a theoretical foundation for establishing a more effective isolation method.
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