Viability of GV Oocytes Collected from Carcasses Kept at Room Temperature
Author of the article:DU Wen-jing, AN Xing-lan, WANG Chun-sheng, PIAO Shan-hua, AN Tie-zhu*
Author's Workplace：(Northeast Forestry University, College of Life Sciences, Harbin 150040, China)
Key Words：mouse; preserve at room temperature; GV-oocytes; maturation in vitro; fertilization in vitro
Abstract：This study was carried out to investigate whether the GV oocytes from carcasses could be utilized. Carcasses were preserved at 10℃, 15℃, 20℃ and 25℃ for 8 hours (as control), 14 hours, 24 hours, or 48 hours after mice were killed and GV occytes were collected. After maturating in vitro, these oocytes were fertilized in vitro with the general procedure in advance. 2-cell embryos were cultured in vitro, then viability was observed. The results showed the fertilizing rate in vitro of the group for GV oocytes with cumulus cells after maturating which were conserved at 10℃ for 24 h, 15℃ for 14 h, 20℃ for 8 h and 25℃ for 4 h was 14%, 9%, 10% and 10% respectively, and with the advance of storage temperature and extension of storage time, the rate of 2-cell after maturating-fertilizating of the GV oocytes decreased significantly. GV oocytes with normal shape were not obtained in the group which was stored at 20℃ for 24 h and at 25℃ for 14 h; A blastula rates (64%) of all groups in average was gotten in culturing 2-cell in vitro,and there is no significant difference in temperature and time of storage. After transplantating some of the 2-cell embryos which were obtained from GV oocytes stored at 15℃ for 8 h, normal infant mice could be obtained. The results above indicated that if we can collect GV oocytes from dead female mice preserved at room temperature in a short time, followed by IVF, IVM, IVC and embryo transplantation, we could obtain new infants.