Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Tel:+86-28-85410485
Fax:+86-28-85410485
Email:scdwzz@vip.163.com & scdwzz001@163.com
Your Position :Home->Past Journals Catalog->2009 Vol.28 No.6

Observation on Cell Immunity of the Musca domestica Larva Infected by Escherichia coli
Author of the article:YAN Rong, LIU Hui, HE Li-fang, LIU Liu, WAN Qi-hui*
Author's Workplace: (Zunyi Medical College, Zunyi, Guizhou Province 563003, China)
Key Words:Musca domestica larva;Escherichia coli; cell immunity
Abstract:Objective To explore the cell immunity of the 3rd stage larva of Musca domestica after being infected by Escherichia coli. Methods (1) To observe changes in the number of total hemocytes (THC) and the proportion morphous of hemocytes of the 3rd stage larva of Musca domestica after being infected by E. coli. (2) The active changes of acid phosphatase (ACP) and catalase (CAT) in the sera of the 3rd stage larva of Musca domestica at different times after being infected by E. coli were assayed by the colorimetric method. (3) The active changes of diphenol oxidase (DPO) in the sera of the 3rd stage larva of Musca domestica at different times after being infected were assayed by PAGE. Results (1) Compared with the control groups, the number of total hemocytes (THC) of infection in the 4 h, 8 h, 16 h, and 24 h groups increased significantly (P<0.01). (2) The proportion of plasmatocytes and granulocytes of the 4 h, 8 h and 16 h groups after infection were significantly increased (P<0.01) and the proportion of sphrulocytes of all time groups after infection were significantly decreased (P<0.01). However, the proportion of prohemocytes and oenocytoids of the all time groups were not obviously different (P>0.05). (3) Vacuolation and morpholysis were found in plasmatocytes after being infected by E. coli. (4) The activities of both phosphatase (ACP) and catalase (CAT) in the sera of the all time groups were much higher than that of the control groups at 4 h, 8 h, 16 h and 24 h (P<0.01), and the peaks of the two enzymes activity appeared at 8 h and 16 h after infection, respectively, then gradually decreased. (5)Diphenol oxidase (DPO) existed in the Musca domestica larva, and its optical density value increased at 4 h after infection, and the peak of optical density value appeared at 16 h, then gradually decreased.  Conclusion Cell immunity of theMusca domestica larva was induced by infection with E. coli, which included a change in the morphology and quantity of hemocytes, and hemocytes could synthesize and release various kinds of enzymes to kill microorganisms.
CopyRight©2022 Editorial Office of Sichuan Journal of Zoology 蜀ICP备08107403号-3