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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2009 Vol.28 No.6

Methods of Sample Preparation for Microdermatoglyphics Research of Squamate Reptiles
Author of the article:REN Dong-yun1,SHI Lei1,2*
Author's Workplace:(1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China; 2. Chengdu Institute of Biology, Chinese Academy of Sciences)
Key Words:squamata; microdermatoglyphics; scanning electric microscope (SEM); sample preparation
Squamate reptiles have a complicated shedding cycle and their epidermis have heterogeneous histological layers, but only the oberhautchen bears the repeating pattern of ornamentation. During the preparation of samples for scanning electron microscopy (SEM), the main source of variation in epidermal surface structure is the confusion of histological layers caused by unidentified stages in the shedding cycle. Using a method of gross dissection for examining preserved specimens to determine the stage in the shedding cycle, we can identify layers dispensing with histological techniques, and characterize their surface. A simple method for preparing the epidermis slice for optical microscopy is also introduced in this paper.
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