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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.2

Characterization of GPR15 Gene in Chicken: Cloning, Functional Analysis and Tissue Distribution
Author of the article:FANG Chao, ZHANG Jiannan, LI Xiaoxiao, CHEN Jun'an, LI Juan, WANG Yajun*
Author's Workplace:Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:chicken; GPR15; functional properties; tissue distribution
Abstract:Orphan receptor G protein-coupled receptor 15 (GPR15) has been reported to play important roles in mammals, including intestinal homeostasis and inflammatory responses. However, the information regarding GPR15 in non-mammals remains unknown. In this study, the coding region of chicken GPR15 was cloned, and its function and tissue expression pattern were also investigated. We found that the coding region of chicken GPR15 was 1 107 bp in length, which was consisted of 1 exon encoding a protein of 368 amino acids. Sequence analysis indicated that chicken GPR15 had a seven-transmembrane structure of GPR, which shared about 56% amino acid sequence identity with GPR15 of human (Homo sapiens), anole lizard (Anolis carolinensis) and mouse (Mus musculus). Although phylogenetic analysis showed that the GPR15 and APLNR were closely related, GPR15 failed to be activated by the endogenic ligands of APLNR (Apelin and Apela peptide). Quantitative real-time PCR assay showed that chicken GPR15 was highly expressed in the spleen, moderately expressed in caecum and lung, and weakly expressed in other remaining tissues. Our data lay a foundation for further uncovering the physiological roles of GPR15 in birds.
2019,38(2): 130-138 收稿日期:2018-05-03
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