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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.1

Characterization of Microsatellites in Phrynocephalus axillaris Genome Using Roche 454 GS FLX
Author of the article:SONG Qi1,2, LIU Jinlong1, GUO Xianguang1*
Author's Workplace:1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China
Key Words:Phrynocephalus axillaris; genome; microsatellite; distribution
Abstract:Yarkand toad-headed agama (Phrynocephalus axillaris), a small reptile endemic to China, is widely distributed in Tarim Basin, Turpan-Hami Basin, Xinjiang Uygur Autonomous Region and Dunhuang Basin, Gansu province. The whole genome of P. axillaris was sequenced using Roche 454 GS FLX platform, and 91 190 high quality sequences were obtained. A total of 29 890 simple sequence repeats with 1-6 bp nucleotide motifs were identified by Krait. Among the different repeat types of the microsatellites, mononucleotide (48.95%) was the most common repeat unit, followed by the di- (28.60%), tetra- (10.73%), tri- (10.48%), penta- (0.92%) and hexanucleotides (0.32%). AC, ATC, AAAT, AAAAT, and AATCCC were the most common repeat units among the di-, tri-, tetra-, penta-, and hexanucleotides, respectively. In the genome of P. axillaris, there were 11 predomiant repeat types, which included C, A, AC, AG, AAAT, ATC, AT, AAT, ATAG, AGG and AAC. This study contributes to the understanding of the genetic feature for P. axillaris, and will provide data support for development and screening of high quality microsatellite markers. In addition, this study lays a foundation for further revealing the population genetic structure and phylogeographic pattern of this lizard using microsatellite markers.
2019,38(1): 62-67 收稿日期:2018-09-03
分类号:Q959.6;R857.3
基金项目:科技部重点研发计划项目(2017YFC0505202);国家自然科学基金项目(31672270)
作者简介:宋琪(1992—),女,硕士研究生,研究方向:分子进化与系统发育,E-mail:songqi@cib.ac.cn
*通信作者:郭宪光,E-mail:guoxg@cib.ac.cn
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