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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.1

Twenty-Five Microsatellite Loci of Ailurus fulgens Identified by Genome Survey
Author of the article:YANG Ailin1,2, XIE Junjin1, SHEN Fujun1, ZHANG Wenping1, HOU Rong1, ZHANG Zhihe1, ZHANG Liang1*
Author's Workplace:1. Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China;
2. Beijing Normal University, Beijing 100875, China
Key Words:Ailurus fulgens; microsatellite locus; population analysis
Abstract:We developed 467 microsatellite loci from a microsatellite-enriched database for red panda (Ailurus fulgens) using the Roche 454 Genome Sequencer Platform. From this database, we isolated and characterized 115 microsatellite loci and chose 25 to genotype in 42 red pandas, with repeat motifs like TAT, GATA, AAACA, AAAACA and so on. The number of observed alleles ranged from 3 to 10. Observed and expected heterozygosities ranged from 0.143 to 0.929 and from 0.136 to 0.869, respectively. The mean polymorphic information content was 0.128-0.843, and the loci were not deviated from expectation of the Hardy-Weinberg equilibrium. We recommend that these markers be used in the systematic control of genetic management for A. fulgens. These markers could also be used as powerful tools applying in the population genetic research of A. fulgens in the future.
2019,38(1): 56-61 收稿日期:2018-06-11
分类号:Q959.838;R318.04
基金项目:科技部国家重点技术研发计划项目(2012BAC01B06);成都市科技管理局项目(12GGYB647SF-002);成都市建设管理委员会研究基金项目;成都市大熊猫繁育研究基金会项目(CPF2017-14)
作者简介:杨艾琳(1989—),博士,研究方向为种群遗传学,E-mail:a285919701@163.com
*通信作者:张亮,E-mail:lianne@panda.org.cn
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