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Issue:ISSN 1000-7083
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.6

Molecular Cloning and Tissue Expression of Chicken Natriuretic Peptide Receptors B Gene
Author of the article:LI Zhengyang, ZHANG Jiannan, CHEN Jun'an, LI Juan, WANG Yajun*
Author's Workplace:Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:NPR-B; gene cloning; functional analysis; tissue expression; physiological function
Abstract:As a selective receptor of C-type natriuretic peptide (CNP), the natriuretic peptide receptor B (NPR-B) regulates various physiological processes, including pituitary hormone secretion, cardiovascular stability, nervous system development, cell proliferation and bone growth. In this study, the full-length cDNA of Gallus gallus domesticus NPR-B gene was reported and the tissue expression of NPR-B gene in adult chickens was detected by quantitative real-time PCR (qPCR). The results showed that the full length of chicken NPR-B gene was 3 189 bp encoding a protein of 1 062 amino acids. The amino acid sequence of NPR-B was highly homologous to that of Homo sapiens (79%), Rattus norvegicus (78%), Xenopus laevis (73%) and Danio rerio (67%). The result of qPCR showed that NPR-B gene was highly expressed in the heart, muscle, midbrain and pituitary of chicken. These findings laid a foundation for further explaining the physiological functions of CNP and NPR-B in chickens.
2018,37(6): 653-659 收稿日期:2018-05-03
分类号:Q959.7;Q78
基金项目:国家自然科学基金项目(31771375)
作者简介:李正阳(1991—),硕士,主要从事动物分子遗传学研究,E-mail:lzy1991716@outlook.com
*通信作者:王亚军,E-mail:cdwyjhk@gmail.com
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