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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.4

Analysis on the Intestinal Microbiota Diversity of Adult Periplaneta americana
Author of the article:HAO Yanqin1, ZHOU Chuang1, JIN Jiazheng1, SHEN Yongmei2, ZHANG Xiuyue1, YUE Bisong1*
Author's Workplace:1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Key Laboratory of Medicinal American Cockroach, Chengdu 610081, China
Key Words:Periplaneta americana; intestinal microbiota; high-throughput sequencing
Abstract:Periplaneta americana is not only an important pest affecting human health, but also a medicinal economic insect. Many studies have suggested that the type and quantity of intestinal microbiota are closely related to the host health. In this study, 16S rRNA high-throughput sequencing technique was used to analyze the intestinal microbiota of artificially fed P. americana. The results showed that a total of 67 492 16S rRNA sequences classified as 581 operational taxonomic units, annotated to 193 genera, 79 orders and 21 phyla, were obtained. Specifically, among the identified species in the intestinal microbiota of P. americana, Firmicutes (56.96%) and Bacteroidetes (33.13%) were the dominant phyla, Bacteroidales (32.11%), Bacillales (29.89%), Clostridiales (12.93%) and Lactobacillales (10.73%) were the dominant orders, Staphylococcus (16.59%), Bacillus (13.26%) and Parabacteroides (10.26%) were the dominant genera, and Staphylococcus sciuri (14.95%) was the most prevalent species. These results laid a solid foundation for further exploring the intestinal microbiota diversity and its relationship with the host P. americana.
2018,37(4): 415-419 收稿日期:2018-03-12
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