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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.4

Application of DNA Barcoding in Identification of Encyrtid Parasitoids of Acanthococcus (=Eriococcus) lagerstroemiae
Author of the article:ZHANG Zeyuan1, QIN Yaoguang2, LI Shuqiang2*
Author's Workplace:1. Division of the High School Affiliated to Renmin University of China, Beijing 100080, China;
2. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Key Words:encyrtid parasitoids; mitochondrial COⅠ gene; species identification; Acanthococcus (=Eriococcus) lagerstroemiae
Abstract:Acanthococcus (=Eriococcus) lagerstroemiae is an important pest of Punica granatum L. and Lagerstroemia indica L. and broadly distributed in China. Encyrtid parasitoids play a key role in the control of A. (=Eriococcus) lagerstroemiae. We obtained samples of the encyrtid parasitoids by rearing them from nymphs of A. (=Eriococcus) lagerstroemiae collected from different areas of China. DNA barcoding approach was applied to the identification of encyrtid parasitoids and followed by a preliminary morphological identification. DNA sequence data of the mitochondrial COⅠ gene were generated from 93 individuals of encyrtid parasitoids. The results showed that the inter-specific COⅠ divergence was 0.073 2~0.186 4 with an average of 0.111 0; and the intra-specific COⅠ variation was 0~0.026 5 with an average of 0.007 4. The inter-specific COⅠ divergence was greatly larger than intra-specific COⅠ variation in the encyrtid parasitoid species. Our results indicated that, as a complementary approach to morphology, DNA barcoding can help us to rapidly and accurately identify the encyrtid species.
2018,37(4): 406-414 收稿日期:2017-09-21
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