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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.3

Genetic Diversity of Offsprings Derived from Wild and Cultivated Populations of Macaca thibetana
Author of the article:NIU Lihong1, ZHOU Liang1, YANG Fan1, WU Aiming1, FU Guoqiang1, HOU Linlin1, LI Xiao2, DOU Kefeng2, CHEN Hui1, ZHANG Xinshang1*
Author's Workplace:1. Institute of Laboratory Animal Sciences, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610212, China;
2. Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China
Key Words:Macaca thibetana; generation; mitochondrial DNA; D-loop; genetic diversity
Abstract:To evaluate the genetic variation of offsprings derived from wild and cultivated populations of Macaca thibetana, we analyzed the whole sequence of mitochondrial DNA D-loop region in the 102 individuals of 2 generations of wild (Jinyang region) and cultivated populations (Mabian region). The whole length of the region sequence was 1 091 bp and 115 variable sites were detected. The 102 individuals presented 66 haplotypes, and the 2 generations of Mabian and Jinyang population shared 2 and 1 haplotype with their parents, respectively. The nucleotide polymorphisms of the 2 generations of Mabian population were 0.004 77 and 0.004 09, while those in Jinyang population were 0.002 30 and 0.002 78, indicating no significant difference of genetic diversity occurred between the offsprings and parents of M. thibetana. Genetic distances between the 2 generations of Mabian and Jinyang populations were 0.024 09 and 0.002 55, indicating no significant differentiation happened in the 2 generations in wild or in cultivated situation. NJ tree based on the whole length of D-loop showed 2 independent clades existed in Mabian and Jinyang populations with different generations crossed each other, this demonstrated that neither the random mating nor the artificial selection pressure exerted a significant influence on the genetic structure of the progeny. This study was the first time to use the whole length of mitochondrial DNA D-loop region to analyze the genetic diversity of wild and cultivated Macaca thibetana populations and their offsprings. Our data provide basic support for the standardization of genetic control of experimental animals.
2018,37(3): 280-284,290 收稿日期:2017-12-13
DOI:10.11984/j.issn.1000-7083.20170394
分类号:Q959.8
基金项目:国家重点基础研究发展计划项目(973计划,2015CB554100)
作者简介:牛丽红(1984-),女,硕士,主要研究非人灵长类疾病动物模型,E-mail:382752406@qq.com
*通讯作者:张新尚,男,博士,主要从事非人灵长类动物保护及利用研究,E-mail:sanmaosound@163.com
参考文献:
黄雪贞, 钱国英, 李彩燕. 2012. 中华鳖3个地理群体线粒体基因D-loop区遗传多样性分析[J]. 水产学报, 36(1):18-24.
蒋学龙, 王应祥, 王歧山. 1996. 藏酋猴的分类与分布[J]. 动物学研究, 17(4):361-369.
李生强, 汪国海, 施泽攀, 等. 2017. 广西藏酋猴种群数量、分布及威胁因素的分析[J]. 广西师范大学学报(自然科学版), 35(2):126-132.
陶开山, 李霄, 窦科峰. 2017. 异种肝移植的历史与发展[J]. 器官移植, 8(2):86-88.
王红星, 姚方, 周亮. 2009. 藏酋猴人工繁殖研究概况[J]. 中国科技成果, 23:38-40.
谢振宇, 杜继曾, 陈学群, 等. 2006. 线粒体控制区在鱼类种内遗传分化中的意义[J]. 遗传, 28(3):362-368.
杨锋, 王红星, 周亮, 等. 2010. 藏酋猴外周血细胞及血清生化指标测定与分析[J]. 四川动物, 29(6):1000-1001.
姚永芳, 钟丽菁, 刘家斌, 等. 2013. 峨眉山藏酋猴mtDNA控制区序列变异及种群遗传多样性[J]. 四川动物, 32(6):801-807.
Li D, Fan L, Ran J, et al. 2008. Genetic diversity analysis of Macaca thibetana based on mitochondrial DNA control region sequences[J]. Mitochondrial DNA, 19(5): 446-452.
Notter DR. 1999. The importance of genetic diversity in livestock populations of the future[J]. Journal of Animal Science, 77(1): 61-69.
Yi Y, Zeng T, Zhou L, et al. 2012. Expreimental Tibetan monkey domestication and its application for intraocular pressure measurement[J]. International Journal of Ophthalmology, 5(3): 277-280.
Zhong LJ, Zhang MW, Yao YF, et al. 2013. Genetic diversity of two Tibetan macaque (Macaca thibetana) populations from Guizhou and Yunnan in China based on mitochondrial DNA D-loop sequences[J]. Genes & Genomics, 35(2): 205-214.
Zhou H, Li DQ, Zhang YG, et al. 2006. Study on mitochondrial DNA genetic diversity of Tibetan antelope[J]. Heredias, 28(3): 299-305.
Zhu D, Jamikson BG, Hugall A, et al. 1994. Sequence evolution and phylogenetic signal in control-region and cytochrome b sequences of rainbow fishes (Melanotaeniidae)[J]. Molecular Biology & Evolution, 11(4): 672-683.
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