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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.2

Reproduction and Artificial Domestication of Microhyla fissipes
Author of the article:WANG Xungang1,2, WANG Shouhong1,2, LI Yulong1,2, ZHANG Meihua1,2, ZHANG Mengjie1,3, LIU Lusha1*, JIANG Jianping1*
Author's Workplace:1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. School of Life Sciences, Nanjing Normal University, Nanjing 210046, China
Key Words:Microhyla fissipes; artificial domestication; reproduction
Abstract:In this study, artificial domestication (feeding environment and food selection) and breeding (reproductive behavior and artificial induced spawning) of Microhyla fissipes were carried out. It was observed that the reproduction of M. fissipes started from March and ended in October, and the reproduction-peak was from May to August; one couple could reproduce 5 times per year and the shortest interval between 2 reproductions was 16 days; the size of egg clutch ranged from 15 to 1 100 eggs (310 eggs±203 eggs, n=98), and this was positively correlated with the body mass of female (R2=0.44, P<0.001, n=76). Furthermore, we constructed the optimal domestication system, of which the food was consisted of cooked egg yolk and spirulina powder for tadpoles, springtails for froglets, and fruit flies (Drosophila melanogaster) for subadult and adult frogs. The achievements could help to provide sufficient experimental materials as model animals, and also provide references for the artificial domestication of other frogs and toads.
2018,37(2): 197-201 收稿日期:2017-10-12
DOI:10.11984/j.issn.1000-7083.20170310
分类号:Q959.5
基金项目:中国科学院重点部署项目(KJZD-EW-L13);国家自然科学基金项目(31471964);中国科学院西部青年学者项目(B类)(Y6C3021)
作者简介:王循刚,男,硕士研究生,研究方向:两栖爬行动物系统进化与保育,E-mail:wangxg@cib.ac.cn
*通讯作者:刘露莎,E-mail:liuls@cib.ac.cn;江建平,E-mail:jiangjp@cib.ac.cn
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