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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.2

An Ultra Performance Liquid Chromatography Based Method to Quantity Determine Protocatechuic Acid in Ethanol Extract of Periplaneta americana
Author of the article:GUO Xin1, GONG Rongyan1, ZHU Juanjuan1, ZENG Chengjuan2, SHEN Yongmei2, YUE Bisong1, LI Jing1*
Author's Workplace:1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Medicinal Animals Engineering Research Center, Sichuan Good Doctor Panxi Pharmaceutical Co. Ltd., Chengdu 610031, China
Key Words:Periplaneta americana; protocatechuic acid; ultra performance liquid chromatography
Abstract:The present study reported an ultra performance liquid chromatography (UPLC) based method to quantity determine the protocatechuic acid in the ethanol extract of Periplaneta americana. The column was washed with Waters ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm). The salt solution (containing 0.1% formic acid, 25 mmol ammonium formate) was used as the mobile phase A, and 100% acetonitrile at a flow rate of 0.5 mL·min-1 was used as the mobile phase B. The specimen temperature was 25℃, and column temperature was 40℃. Three detection wavelengths were set at 190-450 nm, 254 nm and 360 nm, respectively. The protocatechuic acid peak at a retention time of 3.3 min with a base line separation was detected. The protocatechuic acid showed a linear relationship (R2=0.999 9) in the linear range, and the average recovery ranged from 98.30% to 99.57% with the relative standard deviation<1%. The content of protocatechuic acid in the ethanol extract from P. americana was quantity determined to be 51.38 μg·mL-1. The establishment of UPLC method to determine the content of protocatechuic acid will facilitate the quality control of P. americana basic productions in future.
2018,37(2): 185-190 收稿日期:2017-07-07
DOI:10.11984/j.issn.1000-7083.20170216
分类号:R286
作者简介:郭昕(1992-),女,硕士研究生,研究方向:农业昆虫与害虫防治,E-mail:1534657074@qq.com
*通讯作者:李静,E-mail:ljtjf@126.com
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