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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.2

DNA Barcoding of Bambusa emeiensis-Feeding Leafhoppers in China for Species Identification
Author of the article:YAO Yalin1,2,3,4, LUO Qiang2,3,4, DONG Mengshu2,3,4, CHEN Xiangsheng2,3,4, YANG Lin2,3,4*
Author's Workplace:1. College of Forestry, Guizhou University, Guiyang 550025, China;
2. Special Key Laboratory for Development and Utilization of Insect Resources of Guizhou University, Guiyang 550025, China;
3. Institute of Entomology, Guizhou University, Guiyang 550025, China;
4. Key Laboratory for Plant Pests Management of Mountainous Region in Guizhou Province, Guiyang 550025, China
Key Words:DNA barcoding; COⅠ; 16S rRNA; species identification; leafhoppers; Bambusa emeiensis
Abstract:Leafhoppers play a key role in biodiversity and plant protection of agricultural and forestry ecosystems. However, species identification of leafhoppers is a challenge for this subject due to their morphology diversity. DNA barcoding provides an efficient method for species identification. In the present study, partial COⅠ and 16S rRNA gene sequences from 12 Bambusa emeiesis-feeding leafhopper species in China were amplified. A neighbor-joining phylogenetic tree was constructed based on Kimura 2-parameter (K2P) genetic distance, and further studied using Klee-diagram vector analysis. Intra-specific K2P genetic distance averaged 0.004 for COⅠ, and 0.003 for 16S rRNA; inter-specific K2P genetic distance averaged 0.283 and 0.257 for COⅠ and 16S rRNA, respectively. An obvious ‘barcoding gap’ was detected for all species in both markers, and all individuals were clustered together in both phylogeny trees constructed using COⅠ and 16S rRNA. The result of cluster analyses based on COⅠ and 16S rRNA gene sequences were basically consistent with that of morphological identification, and different individuals tested in this study were gathered in a common branch of the dendrograms. Moreover, the result of Klee-diagram vector analysis was also consistent with phylogeny. Hence, both COⅠ and 16S rRNA genes appear to be accurate and efficient markers for bamboo leafhoppers identification in China.
2018,37(2): 127-138 收稿日期:2017-09-23
DOI:10.11984/j.issn.1000-7083.20170294
分类号:Q953;Q969.35
基金项目:国家自然科学基金项目(31260178,31660209);贵州省高层次创新型人才("百"层次)项目(黔科合人才20154021);贵州省节肢动物资源开发利用科技创新人才团队项目(黔科合人才团队[2014]4001号)
作者简介:姚亚林(1990-),男,硕士研究生,主要从事昆虫区系分类和农林昆虫物种多样性研究,E-mail:yaoyalin08@sina.com
*通讯作者:杨琳,E-mail:yanglin6626@163.com
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