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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.1

Molecular Cloning and Tissue Expression of Chicken GPR119-like Gene
Author of the article:DENG Qiuyang, ZHANG Jiannan, WANG Yajun, LI Juan*
Author's Workplace:Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:chicken; GPR119b gene; molecular cloning; tissue expression
Abstract:G protein-coupled receptor 119 (GPR119) is a member of class A (rhodopsin-type) G protein-coupled receptor family. In mammals, GPR119 gene is found to be highly expressed in pancreatic islet β cells, PP cells and intestine endocrine L cells involving in insulin secretion. In this study, we cloned a chicken GPR119-like gene (named cGPR119b) from intestine and examined its expression in chicken tissues. The results showed that the full-length cDNA of cGPR119b was 954 bp in length and encoding a precursor protein of 317 amino acids. Sequence analysis revealed that chicken GPR119b had high amino acid sequence identity with that of Anas platyrhynchos (79.9%), Chelonia mydas (61.0%), Chrysemys picta bellii (62.3%), and Meleagris gallopavo (95.5%). 5'-RACE PCR assay revealed that the 5'-UTR of cGPR119b is 678 bp. Quantitative real-time PCR assay showed that cGPR119b gene was widely expressed in various chicken tissues including the brain, liver, kidney, intestine, ovary, testis, pituitary, pancreas, skin, fat, lung, duodenum, jejunum, ileum, cecum and rectum. cGPR119b gene was highly expressed in liver, kidneys, cecum and testes, whereas weakly expressed in the pancreas, skin, fat and lung. The present study provided a basis to uncover the physiological roles of cGPR119b gene in chickens.
2018,37(1): 45-50 收稿日期:2017-05-02
DOI:10.11984/j.issn.1000-7083.20170142
分类号:Q959.7;Q78
作者简介:邓秋洋(1990-),硕士,主要从事动物分子遗传学研究,E-mail:13689043376@163.com
*通讯作者:李娟,E-mail:lijuanscuhk@163.com
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