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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.5

Analysis of Repetitive Sequences in Periplaneta americanana Genome
Author of the article:MOU Biqin1, YAN Chaochao1, LI Wujiao1, LI Jing1, SHEN Yongmei2, YUE Bisong1*
Author's Workplace:1. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China;
2. Sichuan Key Laboratory of Medicinal Periplaneta americana, Chengdu 610081, China
Key Words:Periplaneta americana; genome; repetitive sequences; transposable element; BovB retrotransposon; phylogeny
Abstract:Repetitive sequences constitute a large fraction of a eukaryote genome. Some classes of repetitive sequences, such as LINEs, which are a member of autonomous transposons, have been widely applied in molecular phylogenetic and genetic diversity studies of insects. In this study, de novo prediction and homology alignment were used to search the repetitive sequences in Periplaneta americana genome, and the results showed that the screened repetitive sequences accounted for approximately 62% of the genome. Among interspersed repetitive sequences, the DNA transposons constituted 16.18% of the whole genome. In retrotransposons, LINEs accounted for 13.64% of the genome followed by SINE (3.52%) and LTR (1.32%), respectively. The BovB superfamily in LINEs was the most abundant (6.73%) compared to other classes of transposons.The proportion of all classes of transposable elements in P. americana were higher than that of Blattella germanica except the LTRs. According to the integrality of the reverse transcriptase of retrotransposons, similarities and genetic distance of the amino acid sequences, a kind of BovB retrotransposons were identified in P. americana genome and named RTE-1_PAm. Phylogenetic analyses based on the amino acid sequences of BovB reverse transcriptase indicated that P. americana shared closer relationship with Zootermopsis nevadensis than with B. germanica which are both Blattidae. The difference of phylogenetic analyses results among these insects inferred by using retrotransposons and karyogene suggested an independent evolutional history of TEs relative to the host karyogene.
2017,36(5): 540-547 收稿日期:2017-02-22
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