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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.3

Anti-fertility Effect of Peppermint Oil on Female Lasiopodomys brandtii
Author of the article:ZHANG Xingli, XIE Chenwen, LIU Xiaoxue, FANG Wei, LI Xiao, ZHANG Jie*
Author's Workplace:College of Life Sciences, Sichuan University, Chengdu 610064, China
Key Words:Lasiopodomys brandtii; peppermint oil; embryo implantation; anti-fertility
Abstract:The occurrence area of grassland rodents in China has been increasing year by year. Brandt's vole (Lasiopodomys brandtii) is a major rodent grassland pest found in Inner Mongolia with high breeding rate. In order to find a more effective way to control the number of rodents, the anti-fertility effect of peppermint oil on Brandt's voles was investigated. The non-overwintering mature female and male Brandt's voles were mated in a 2:1 ratio. Then the pregnant voles were randomly divided into 3 treatment groups (the concentrations of peppermint oil were:750 mg·kg-1, 1 000 mg·kg-1 and 1 500 mg·kg-1, respectively) and 1 control group. After intragastric administration for 7 days, the voles were then killed for further autopsy. The number of implanted uterus was counted, and the histopathological study of uterus was carried out. The results showed that peppermint oil could reduce the number of implanted embryos in Brandt's voles. The result of scanning electron microscopy showed that the endometrial receptivity of Brandt's voles administrated by peppermint oil was changed. Histopathological results showed that the uterus morphology of Brandt's voles treated with peppermint oil was also changed. Moreover, the immunohistochemical results showed that the expression levels of estrogen receptor α (ERα), progesterone receptor (PR), matrix metalloproteinase-2 (MMP2), and E-cadherin were all abnormal. These findings showed that the anti-fertility effect of peppermint oil on Brandt's voles was mainly through regulating the expression level of ERα, PR, MMP2 and E-cadherin to affect endometrial receptivity and embryo implantation, and finally led to implantation failure. Therefore, the peppermint oil can be used as a potential plant-derived infertility in rodent sterility control.
2017,36(3): 317-324 收稿日期:2016-10-26
DOI:10.11984/j.issn.1000-7083.20160292
分类号:Q959.837;Q95-33
基金项目:公益性行业(农业)科研专项项目(201203041);四川省科技支撑计划项目(2012SZ0045)
作者简介:张兴利(1991-),女,硕士研究生,研究方向:植物源性鼠不育剂的筛选,E-mail:1258900385@qq.com
*通讯作者:张杰,E-mail:zhangjfy@163.com
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