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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.3

Study on Preventive Effect of Cecropin and Lysozyme on Skin Fester Disease of Trionyx sinensis
Author of the article:CHEN Chunshan, GUO Minglei, WEI Kai, MA Long, GENG Yan
Author's Workplace:Beijing Aquatic Wildlife Rescue and Conservation Center, Beijing 102100, China
Key Words:Trionyx sinensis; cecropin; lysozyme; skin fester disease; prevention
Abstract:In order to study the preventive effect of peptides and biological antibiotics on the skin fester disease of Trionyx sinensis caused by Aeromonas encheleia, 3 different groups of cecropin (10 mg·kg-1), lysozyme (10 mg·kg-1) and mixed drugs (6 mg·kg-1 cecropin and 5 mg·kg-1 lysozyme, respectively) were used for intramuscular injection. The mortality rate and relative percent survival were then calculated to evaluate the protective effects of different drugs. Agglutination titer of serum, phagocytosis and bactericidal activity of serum were measured to compare the effects of different drugs on immunity and disease resistance of T. sinensis. The residual concentrations of gentamicin and lysozyme were compared to evaluate drug toxic and side effects. The results showed that mixed drugs could extremely significant (P<0.01) protect T. sinensis and increase their immunity. Analysis of drug residue showed that lysozyme was completely metabolized after 6 days, and in contrast, gentamicin still had residue until day 12 after withdrawal. Therefore, the preventive effect of mixed drugs is significantly better than antibiotics in skin fester disease of T. sinensis. In addition, mixed drugs of lysozyme and cecropin are worthy of spreading to application in virtue of no drug residue and other side effects, especially avoiding the damage caused by abuse of antibiotics.
2017,36(3): 311-316 收稿日期:2016-12-24
DOI:10.11984/j.issn.1000-7083.20160368
分类号:Q959.6;S858.9
基金项目:北京市农业科技试验示范项目(20110821)
作者简介:陈春山(1962-),男,高级工程师,研究方向:水生野生动物保护技术,E-mail:chenchunshan8888@126.com
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