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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.3

Comparison of mRNA Levels of PPP2CA and PME-1 Genes in the Testes of Yaks and Sterile Cattle-yaks
Author of the article:REN Liang1,2, FU Wei1, JIN Suyu1, HUANG Lin1, LI Caixia1, ZHENG Yucai1*
Author's Workplace:1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;
2. Jiange Animal Disease Control and Prevention Centre, Guangyuan, Sichuan Province 628300, China
Key Words:yak; cattle-yak; hybrid male sterility; protein phosphatase 2A; protein methylesterase 1
Abstract:Protein phosphatase 2A (PP2A) mainly functions in protein reversible phosphorylation, and plays a central role in phosphorylation-dependent signaling pathways. Its activity is regulated by protein methylesterase 1 (PME-1) and other factors. To elucidate the molecular mechanism of hybrid male sterility of yaks, we cloned cDNA sequences of catalytic subunit α (PPP2CA) of PP2A and PME-1 genes. The coding regions of yak PPP2CA and PME-1 genes were 930 bp and 1 143 bp, respectively. The deduced amino acid sequences were conserved. Quantitative PCR assay showed that cattle-yaks (n=7) contained dramatically reduced mRNA levels of PPP2CA and PME-1 genes in testes than yaks (n=13, P<0.01). The present results suggested that the reduced mRNA levels of the two genes in the testes might influence some important signaling pathways and associate with hybrid male sterility of cattle-yaks.
2017,36(3): 306-310 收稿日期:2016-11-30
DOI:10.11984/j.issn.1000-7083.20160337
分类号:Q311
基金项目:国家自然科学基金项目(31240053)
作者简介:任亮(1988-),助理兽医师,硕士,研究方向:生化分析及分子生物学方向
*通讯作者:郑玉才,教授,博士,E-mail:yucaizheng65@hotmail.com
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