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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.3

Effects of Cu2+ Stress on Hemocytes Toxicity and caspase-3, α-2M Gene Expression in Macrobrachium rosenbergii
Author of the article:GUO Hui, ZHU Xiaowen, OU Ronghua, LU Zhicheng, TAN Cuiting, SHEN Yuchun, ZHU Chunhua*
Author's Workplace:Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong Province 524025, China
Key Words:Macrobrachium rosenbergii; Cu2+; hemocytes toxicity; gene expression; apoptosis
Abstract:The present study analyzed the toxic effects of Cu2+ on apoptotic cell ratio, ROS production, esterase activities, NO production, and the expression levels of caspase-3 and α-2M in hemocytes of Macrobrachium rosenbergii. The results showed that the changes of apoptotic cell ratio were consistent with ROS production which was increased significantly (P<0.05) after exposure for 6~48 h. Esterase activities were significantly decreased (P<0.05) at 3~48 h and the minimum was observed at 48 h. The NO production continued increasing and the level became significant difference (P<0.05) after exposure for 6~48 h. The relative expression levels of caspase-3 were significantly induced (P<0.05) after exposure for 6~48 h, and reached the peak at 24 h. The relative expression of α-2M was significantly increased (P<0.05) and reached the peak at 12 h. Moreover, the expression level of α-2M reduced after exposure for 24 h and became significantly different(P<0.05) at 48 h. Correlation analysis showed that significant positive correlation (P<0.001) between apoptotic cell ratio and ROS production was observed, as well as apoptotic cell ratio and NO production. All these results suggested that esterase activities were significantly inhibited after Cu2+ exposure, while NO and ROS production as well as α-2M expression were induced against Cu2+ exposure. Along with stress time increasing, overfull production of ROS and NO caused oxidative stress to prawn and then induced the expression level of caspase-3 which finally resulted in apoptosis, indicating that Cu2+ stress may have immunosuppressive and toxic effects to M. rosenbergii, and the increased production of NO and ROS might be the main cause of apoptosis.
2017,36(3): 293-299 收稿日期:2017-02-08
DOI:10.11984/j.issn.1000-7083.20170035
分类号:X503.225;Q959.223
基金项目:国家自然科学基金项目(31600321);广东省自然科学基金项目(2015A030310438);广东海洋大学创新强校工程科研项目(GDOU2016050253);广东海洋大学优秀青年骨干教师特别资助计划项目(HDYQ2015003)
作者简介:郭慧(1986-),女,讲师,博士,主要从事水产动物健康养殖生态学研究,E-mail:guohuivivian@163.com
*通讯作者:朱春华,男,教授,主要从事水域生态与养殖环境研究,E-mail:zhu860025@163.com
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