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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.3

Analysis of Genetic Diversity among Different Geographic Populations of Anaka burmensis (Hemiptera: Cicadellidae) Based on Part of mtDNA CO Ⅰ Gene Sequences
Author of the article:DONG Mengshu1, YANG Lin1,2,3*, CHEN Xiangsheng1,2,3, ZHANG Yujie1
Author's Workplace:1. Institute of Entomology, Guizhou University, Guiyang 550025, China;
2. Guizhou Key Laboratory for Plant Pest Management of Mountainous Region, Guiyang 550025, China;
3. Special Key Laboratory of Insect Resource Development and Utilization, Guiyang 550025, China
Key Words:Anaka burmensis; mtDNA CO Ⅰ gene; geographic population; genetic diversity
Abstract:Anaka burmensis is an important pest which feed on bamboo. To investigate the genetic variation of A. burmensis among different geographic populations in China, the 615 bp segments of the mitochondrial cytochrome coxidase subunit Ⅰ (CO Ⅰ) gene of A. burmensis individuals from 26 geographic populations were analyzed by using DNASP, MEGA, etc. The results showed that there were 546 conserved sites, 69 mutation sites, 33 haplotypes in the fragments; a high level of genetic diversity (haplotype diversity index:0.845; nucleotide diversity index:0.008 77) in the total population was detected. Additionally, high genetic differentiation (fixation index:0.729 15) was found among different geographic populations but with low gene flow level (0.598 5). The differences of the Neutral test (Tajima's D=-1.658 98, 0.10 > P > 0.05, Fu's Fs=-5.787, P>0.10) were not significant. The result of molecular variance analysis showed that the genetic differentiation among populations was 72.92% and higher than that of within populations (27.08%). Therefore, here we concluded the genetic structure of A. burmensis can provide a theoretical basis for future research of molecular biology and the control of this insect.
2017,36(3): 277-283 收稿日期:2017-01-21
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