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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.2

Expression of mEppin Gene in Different Developing Stages of Male Mice Testes
Author of the article:TANG Donghong1, YE Yousong1, LI Zheli1, LI Si2, YANG Guangrui2, LI Guizhen2, LI Runping2, PENG Bo2
Author's Workplace:1. Institute of Medical Biology, Chinese Academy of Medical Science/Peking Union Medical College, Kunming 650118, China;
2. Yunnan University of Traditional Chinese Medicine, Kunming 650200, China
Key Words:mouse; mEppin gene; different mRNA expression; real-time fluorescent quantitative PCR
Abstract:Objective The expression of mEppin gene in different developing stages of male mice testes was examined by real time fluorescent quantitative PCR (qPCR) assay. This study aims to provide some informations regarding the association of mEppin gene expression with male reproduction. Methods The total RNA was extracted from testes of 18 ICR mice at different developing stages (embryo, 50 d-old, and 90 d-old). The expression levels of mEppin mRNA in testis were detected by qPCR. Results The expression levels of mEppin gene among three developing stages of testes were significantly different. High expression levels of mEppin gene were detected in the testes of 50 d-old and 90 d-old mice, whereas no expression was detected in embryonic testes. The expression level of mEppin gene in the testis of 90 d-old mice was 2.65-fold higher than that in 50 d-old. Conclusion The mEppin gene expression in developing testes is stage-dependent in mice.
2016,(35): 256-260 收稿日期:2015-09-02
作者简介:唐东红(1966-), 女, 主任技师, 研究方向:疾病动物模型,,
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