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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.2

Expression of mEppin Gene in Different Developing Stages of Male Mice Testes
Author of the article:TANG Donghong1, YE Yousong1, LI Zheli1, LI Si2, YANG Guangrui2, LI Guizhen2, LI Runping2, PENG Bo2
Author's Workplace:1. Institute of Medical Biology, Chinese Academy of Medical Science/Peking Union Medical College, Kunming 650118, China;
2. Yunnan University of Traditional Chinese Medicine, Kunming 650200, China
Key Words:mouse; mEppin gene; different mRNA expression; real-time fluorescent quantitative PCR
Abstract:Objective The expression of mEppin gene in different developing stages of male mice testes was examined by real time fluorescent quantitative PCR (qPCR) assay. This study aims to provide some informations regarding the association of mEppin gene expression with male reproduction. Methods The total RNA was extracted from testes of 18 ICR mice at different developing stages (embryo, 50 d-old, and 90 d-old). The expression levels of mEppin mRNA in testis were detected by qPCR. Results The expression levels of mEppin gene among three developing stages of testes were significantly different. High expression levels of mEppin gene were detected in the testes of 50 d-old and 90 d-old mice, whereas no expression was detected in embryonic testes. The expression level of mEppin gene in the testis of 90 d-old mice was 2.65-fold higher than that in 50 d-old. Conclusion The mEppin gene expression in developing testes is stage-dependent in mice.
2016,(35): 256-260 收稿日期:2015-09-02
DOI:10.11984/j.issn.1000-7083.20150268
分类号:Q78
作者简介:唐东红(1966-), 女, 主任技师, 研究方向:疾病动物模型, E-mail:tdh@imbcams.com.cn, 783849021@qq.com
参考文献:
王增军, 吴宏飞, 钱立新, 等. 2006. 人类精子表面附睾蛋白激酶抑制剂Eppin和精囊凝固蛋白Semenogelin的相互作用研究[J]. 中华男科学杂志, 12(5): 428-431, 434.
Denolet E, De Gendt K, Allemeersch J, et al. 2006. The effect of a sertoli cell-selective knockout of the androgen receptor on testicular gene expression in prepubertal mice[J]. Molecular Endocrinology, 20(2): 321-334.
Lim P, Robson M, Spaliviero J, et al. 2009. Sertoli cell androgen receptor DNA binding domain is essential for the completion of spermatogenesis[J]. Endocrinology, 150(10): 4755-4765.
Long Y, Gu A, Yang H, et al. 2011. Distribution of Eppin in mouse and human testis[J]. Molecular Medicine Reports, 4(1): 71-75.
O'Rand MG, Widgren EE, Beyler S, et al. 2009. Inhibition of human sperm motility by contraceptive anti-eppin antibodies from infertile male monkeys: effect on cyclic adenosine monophosphate[J]. Biology of Reproduction, 80(2): 279-285.
O'Rand MG, Widgren EE, Sivashanmugam P, et al. 2004. Reversible immunocontraception in male monkeys immunized with eppin[J]. Science, 306(5699): 1189-1190.
O'Rand MG, Widgren EE, Wang Z, et al. 2006. Eppin: an effective target for male contraception[J]. Molecular and Cellular Endocrinology, 250(1-2): 157-162.
O'Rand MG, Widgren EE, Wang Z, et al. 2007. Eppin: an epididymal protease inhibitor and a target for male contraception[J]. Society of Reproduction and Fertility Supplement, 63: 445-453.
Richardson RT, Sivashanmugam P, Hall SH, et al. 2001. Cloning and sequencing of human Eppin: a novel family of protease inhibitors expressed in the epididymis and testis[J]. Gene, 270(1-2): 93-102.
Schauwaers K, De Gendt K, Saunders PT, et al. 2007. Loss of androgen receptor binding to selective androgen response elements causes a reproductive phenotype in a knockin mouse model[J]. Proceedings of the National Academy of Sciences of the United States of America, 104(12): 4961-4966.
Sivashanmugam P, Hall SH, Hamil KG, et al. 2003. Characterization of mouse Eppin and a gene cluster of similar protease inhibitors on mouse chromosome 2[J]. Gene, 312: 125-134.
Willems A, De Gendt K, Allemeersch J, et al. 2010. Early effects of Sertoli cell-selective androgen receptor ablation on testicular gene expression[J]. International Journal of Andrology, 33(3): 507-517.
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