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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2015 Vol.34 No.4

Molecular Cloning of Antifreeze Protein Gene from Oodescelis chinensis (Coleoptera: Tenebrionidae) and Effect of Temperature on Its Expression
Author of the article:ZHANG Fengjuan, WANG Yan, WANG Lu, LI Zhaoyang, TAO Bolin, MA Ji
Author's Workplace:Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China
Key Words:Oodescelis chinensis; freeze tolerant insect; antifreeze protein; temperature stress; real-time fluorescence quantitative PCR
Abstract:Oodescelis chinensis (Coleoptera: Tenebrionidae), a rare freeze tolerant insect endemic to China, distributes in the desert and semi-desert regions of Xinjiang, China. However, it was unknown whether O. chinensis contained antifreeze protein gene. In this study, the total RNA of O.chinensis was extracted by using TRIzol reagent. Several sequences of antifreeze protein genes (Ocafp) were amplified by RT-PCR using the universal primers designed according to the homologous sequences of other beetles. The sequencing results showed that the deduced amino acid sequence of OcAFP shared the similar structure feature of other beetles containing the TCTXSXXCXXAX (where X stood for any amino acid) motif. However, there were many different mutations in the conserved TCT motifs, which may be due to the adaptation of O.chinensis to the extreme variation of environmental temperature since O.chinensis living in the soil surface. The results of real-time fluorescence quantitative PCR showed that the expression of Ocafp could be induced at-4 ℃, 5 ℃ and 42 ℃, respectively, but inhibited at 40 ℃. The results indicated that O. chinensis also contained antifreeze proteins, and the variability of the amino acid sequence was higher than that of other freeze avoidant insects. The expression of Ocafp could be induced either at low temperature or at high temperature, and this suggested that OcAFP may also have other functions.
2015,34(4): 518-523 收稿日期:2014-10-30
DOI:10.11984/j.issn.1000-7083.2015.04.007
分类号:Q966;Q78
基金项目:国家大学生创新创业实训项目(XJU-SRT-13165)
作者简介:张凤娟(1990—),女,硕士研究生,研究方向:荒漠昆虫分子生物学
*通讯作者:马纪,E-mail:majiuci@xju.edu.cn
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