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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2015 Vol.34 No.4

Antagonism of Melatonin against Di-(2-ethylhexyl) Phthalate-induced DNA Damage in Mice Testicular Cells
Author of the article:HUANG Wenjie, FENG Wei, LI Yang, CHEN Yu
Author's Workplace:School of Public Health and Health Management, Medicine and Social Development Research Center, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, China
Key Words:di-(2-ethylhexyl) phthalate (DEHP); melatonin; DNA damage; oxidative damage
Abstract:Objective To investigate the antagonistic effect of melatonin against di-(2-ethylhexyl) phthalate (DEHP)-induced DNA damage in mice testicular cells. Methods A total of forty CL57BL/6J mice were randomly divided into 4 groups: control (saline) group, MT (melatonin) treated group, DEHP treated group and MT+DEHP treated group. MT was intraperitoneally injected at the dose of 15 mg·kg-1, and DEHP was orally administrated at the dose of 1000 mg·kg-1 for 30 days. Subsequently, the activities of testicular glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), the content of testicular malondialdehyde (MDA) and 8-hydrxydeoxyguanosine (8-OHdG) were measured. The comet assay was applied to measure the DNA damage. Comet Assay Software Project was conducted to analyze the DNA damage (comet tail length, tail DNA%, tail moment and Olive tail moment) of testicular cells. Results Compared with the control group, the contents of MDA and 8-OHdG in testis tissues were significantly increased; and the activities of GSH-Px and SOD were significantly decreased (P<0.05); the comet tail length, the percentage of tail DNA, tail moment and Olive tail moment in testicular cells were significantly increased in DEHP group (P<0.05). Compared with the DEHP group, the contents of MDA and 8-OHdG in testis tissues were significantly decreased, the activities of GSH-Px and SOD were significantly increased (P<0.05); and the DNA damage of testicular cells significantly decreased in MT+DEHP group (P<0.05). Conclusion DEHP could cause oxidative stress in testis, and induce DNA damage in mice testicular cells. MT could antagonize DEHP-induced oxidative damage in testis of mice.
2015,34(4): 511-517 收稿日期:2014-10-3
蔡红平, 肖雯, 朱静, 等. 2011. 邻苯二甲酸二乙基己基酯对大鼠睾丸细胞DNA 损伤和睾丸组织氧化应激影响[J]. 中国职业医学, 38(6): 458-462.
崔月美, 叶建新, 吴茂聪, 等. 2009. 邻苯二甲酸酯对小鼠雄性生殖系统的毒性作用[J]. 大连医科大学学报, 31(4): 260-263.
李丽萍, 刘秀芳, 王桂燕. 2008. 邻苯二甲酸(2-乙基己基)酯低剂量暴露对雄性小鼠生殖发育的影响[J]. 环境与健康杂志, 25(40): 308-310.
屈艾, 汪承润, 薄军, 等. 2005. 稀土元素钬对小鼠 3 种抗氧化酶活力的影响[J]. 环境与职业医学, 22(6): 531-537.
商学军, 黄宇烽, 叶章群, 等. 2004. 褪黑素在活性氧致精子线粒体功能损伤中的保护作用[J]. 中国男科学杂志, 10(8): 604-607.
王黎明, 丁书茂, 吴杨, 等. 2007. 邻苯二甲酸二乙基己酯对小鼠不同脏器DNA的损伤作用[J]. 华中师范大学学报, 41(3): 440-443.
张良, 梁元姣. 2014. 褪黑素参与调节卵巢功能的研究进展[J]. 中华男科学杂志, 20(6): 548-555.
Aversa S, Pellegrino S, Barberi I, et al. 2012. Potential utility of me-latonin as an antioxidant during pregnancy and in the perinatal period[J]. The Journal of Maternal-fetal and Neonatal Medicine, 25(3): 207-221.
Carlstedt F, Jonsson BA, Bornehag CG. 2013. PVC flooring is related to human uptake of phthalates in infants[J]. Indoor Air, 23(1): 32-39.
Cavella M, Liporac V. 2000. Antioxidative effect of melatonin on human spermatozoa[J]. Archives of Andrology, 44(1): 23-27.
Christiansen S, Boberg J, Axelstad M, et al. 2010. Low-dose perinatal exposure to di(2-ethylhexyl)phthalate induces anti-androgenic effects in male rats[J]. Reproductive Toxicology, 30(2): 313-321.
De Iuliis GN, Thomson LK, Mitchell LA, et al. 2009. DNA damage in human spermatozoa is highly correlated with the efficiency of chro-matin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress[J]. Biology of Reproduction, 81(3): 517-524.
Dulcy CP, Singh HK, Preethi J, et al. 2012. Standardized extract of Bacopa monniera (BESEB CDRI-08) attenuates contextual associative learning deficits in the aging rats brain induced by D-galactose[J]. Journal of Neuroscience Research, 90(10): 2053-2064.
Foster PM, Mylchreest E, Gaido KW, et al. 2001. Effects of phthalate esters on the developing reproductive tract of male rats[J]. Human Reproduction Update, 7(3): 231-235.
Howdeshell KL, Wilson VS, Furr J, et al. 2008. Mixture of five phthalate esters inhibits fetal testicular testosterone production in the sprague-dawley rat in a cumulative,dose-additive manner[J]. The Journal of Toxicological Sciences, 105(1): 153-165.
Jiang ZQ, Li Y, Jiang LH, et al. 2013. Hepatoprotective effects of extracts from processed corni fructus against D-galactose-induced liver injury in mice[J]. Journal of Chinese Medicinal Materials, 36(1): 85-89.
Kamrin MA. 2009. Phthalate risks, phthalate regulation, and public health:a review[J]. Journal of Toxicology and Environmental Health, Part B:Critical Reviews, 12(2): 157-174.
Koch HM, Lorber M, Christensen KY, et al. 2013. Identigying sources of phthalate exposure with human biomonitoring: results of a 48 h fasting study with urine collection and personal activity patterns[J]. International Journal of Hygiene and Environmental Health, 216(6): 672-681.
Li YF, Pan C, Hu JX, et al. 2013. Effects of rypermethrin on male reproductive system in adult rats[J]. Biomedical and Environmental Sciences, 26(3): 201-208.
Marchetti C, Obert G, Deffosez A, et al. 2002. Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm[J]. Human Reproduction, 17(5): 1257-1265.
Melchiorri D, Reiter RJ, Sewerynek E, et al.1995. Melatonin reduces kainate-induced lipid peroxidation in homogenates of different brain regions[J].The Faseb Journal, 9(12): 1205-1210.
Minaii B, Moayeri A, Shokri S, et al. 2014. Melatonin improve the sperm quality in forced swimming test induced oxidative stress in nandrolone treated Wistar rats[J], Acta, 52(7): 496-504.
Moore RW, Rudy TA, Lin TM, et al. 2001. Abnormalities of sexual development in male rats with in utero and lactational exposure to the antiandrogenic plasticizer di(2-ethylhexyl) phthalate[J]. Environmental Health Perspectives, 109(3): 229-237.
Ortiz A, Espino J, Bejarano I, et al. 2011. High endogenous mela-tonin concentrations enhance sperm quality and short-term in vitro exposure to melatonin improves aspects of sperm motility[J]. Journal of Pineal Research, 50(2): 132-139.
Park JD, Habeebu SM, Klaassen CD. 2002. Testicular to toxicity of di-(2-ethyiliexyl) phthalate in young Sprague Dawley rats[J]. Toxicology, 171(2-3): 105-115.
Reiter RJ, Tan DX, Fuentes-Broto L.2010. Melatonin: a multitasking molecule[J]. Progress in Brain Research, 181: 127-151.
Rozati R, Reddy PP, Reddanna P, et al. 2002. Role of environmental estrogens in the deterioration of male factor fertility[J]. Fertil Steril, 78(6): 1187-1194.
Singh NP, McCoy MT, Tice RR, et al. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells[J]. Experimental Cell Research, 175(1): 184-191.
Wittassek M, Koch HM, Angerer J, et al. 2011. Assessing exposure to phthalates-the human biomonitoring approach[J]. Molecular Nutrition and Food Research, 55(1): 7-31.
Wolfsegger MJ, Jaki T, Dietrich B, et al. 2009. A note on statistical analysis of organ weights in non-clinical toxicological studies[J]. Toxicology and Applied Pacology, 240(1): 117-122.
Xu SC, He MD, Lu YH, et al. 2011. Nickel exposure induces oxidative damage to mitochondrial DNA in Neuro2a cells: the neuroprotective roles of melatonin[J]. Journal of Pineal Research, 51(4): 426-433.
Yu ZH, Chow PH, Pang SF.1994. Identification and characterization of 2[125I]-iodomelatonin binding sites in the rat epididymis[J]. Journal of Pineal Research, 17(4): 195-201.
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