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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2015 Vol.34 No.4

Antagonism of Melatonin against Di-(2-ethylhexyl) Phthalate-induced DNA Damage in Mice Testicular Cells
Author of the article:HUANG Wenjie, FENG Wei, LI Yang, CHEN Yu
Author's Workplace:School of Public Health and Health Management, Medicine and Social Development Research Center, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing 400016, China
Key Words:di-(2-ethylhexyl) phthalate (DEHP); melatonin; DNA damage; oxidative damage
Abstract:Objective To investigate the antagonistic effect of melatonin against di-(2-ethylhexyl) phthalate (DEHP)-induced DNA damage in mice testicular cells. Methods A total of forty CL57BL/6J mice were randomly divided into 4 groups: control (saline) group, MT (melatonin) treated group, DEHP treated group and MT+DEHP treated group. MT was intraperitoneally injected at the dose of 15 mg·kg-1, and DEHP was orally administrated at the dose of 1000 mg·kg-1 for 30 days. Subsequently, the activities of testicular glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), the content of testicular malondialdehyde (MDA) and 8-hydrxydeoxyguanosine (8-OHdG) were measured. The comet assay was applied to measure the DNA damage. Comet Assay Software Project was conducted to analyze the DNA damage (comet tail length, tail DNA%, tail moment and Olive tail moment) of testicular cells. Results Compared with the control group, the contents of MDA and 8-OHdG in testis tissues were significantly increased; and the activities of GSH-Px and SOD were significantly decreased (P<0.05); the comet tail length, the percentage of tail DNA, tail moment and Olive tail moment in testicular cells were significantly increased in DEHP group (P<0.05). Compared with the DEHP group, the contents of MDA and 8-OHdG in testis tissues were significantly decreased, the activities of GSH-Px and SOD were significantly increased (P<0.05); and the DNA damage of testicular cells significantly decreased in MT+DEHP group (P<0.05). Conclusion DEHP could cause oxidative stress in testis, and induce DNA damage in mice testicular cells. MT could antagonize DEHP-induced oxidative damage in testis of mice.
2015,34(4): 511-517 收稿日期:2014-10-3
DOI:10.11984/j.issn.1000-7083.2015.04.006
分类号:R114;R994.6
作者简介:黄文捷(1989—),女,硕士研究生,研究方向:职业人群健康监护和健康促进,E-mail:aibivicky@163.com
*通讯作者:陈于,教授,E-mail:chenyu@cqmu.edu.cn
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