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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.5

COⅠ Sequence Analysis of Scirpophaga incertulas and its Application in DNA Barcoding
Author of the article:MA Wenqi1#, ZOU Yuan1#, YANG Chengzhong2, YUE Bisong1*
Author's Workplace:(1. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Chongqing Key Laboratory of Animal Biology, College of Life Sciences, Chongqing Normal University, Chongqing 400047, China)
Key Words:Scirpophaga incertulas; DNA barcoding; COⅠ
Abstract:Scirpophaga incertulas is one of the important pests affecting rice production. To investigate the genetic differentiation among different populations of S. incertulas in China, the mitochondrial DNA (mtDNA) cytochrome oxidase subunit (CO) of three geographic populations of S. incertulas was sequencedThe identity and variation of the sequences and their phylogenic relationships were analyzed by Mega 5.0. In this study, thirty six individuals of S. incertulas shared nine haplotypes. There were 17 polymorphic loci accounted for 3.13% of the total. The phylogenetic tree revealed no obvious geographical pattern for the prevalence and distribution of the haplotypes. Combining with the sequences of related species published online, genetic analyses were conducted and the results showed that CO sequence can be applied to species identification for S. incertulas.
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