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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.5

Isolation, Identification and Pathogenicity of Aeromonas sobria from Triplophysa siluroides
Author of the article:XU Jingjun, GENG Yi*, WANG Kaiyu, CHEN Cheng, ZHOU Yan, CHEN Defang, HUANG Xiaoli, PU Yundan
Author's Workplace:(College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan Province 625014, China)
Key Words:Triplophysa siluroides; Aeromonas sobria; 16S rRNA; gyrB gene; pathological injury
Abstract:

In September 2013, a serious infectious disease characterized by surface bleeding, cutaneous ulcers, swelling, bleeding and necrosis of visceral organs occurred in the Triplophysa siluroides farm in Ya’an, Sichuanprovince. One Gram-negative, short, rod-shaped bacterial strain (XJJ130928) was isolated from the liver and kidney of sick T. siluroides. The phenotype of the colonies was offwhite, circular, smooth-surface, regular and translucent after incubation at28for 24 h on BHI medium. The strain was considered as the etiological agent as confirmed by artificial infection test. The strain XJJ130928 was primarily identified as Aeromonas sobria according to the morphology, physiological and biochemical characteristics. Furthermore, sequence analysis of the 16S rRNA and gyrB gene by aligning in the GenBank indicated that the 16S rRNA (KF761305) and gyrB gene sequences (KJ139988) of the isolated strain were highly similar to A. sobria. Phylogenetic trees were then constructed by comparing the 16S rRNA and gyrB gene sequences with other homogeneous sequences in GenBank, respectively. In the phylogenetic trees, the isolated strain and other A. sobria strains were clustered into the same branch with 96.0%~99.0% and 94.5%~98.0% sequence similarity. Based on the phenotypic characteristics and phylogenetic analysis of the 16S rRNA and gyrB gene, the isolated strain was confirmed to be A. sobria. The result of drug susceptibility test showed that the strain was sensitive to cefoxitin, imipenem, doxycycline, florfenicol and ofloxacin. Meanwhile, the strain was moderately sensitive to sinomin, iomefloxacin and ciprofloxacin, but resistant to ampiciuin, streptomycin, and kanamycin. Histopathologic observation showed that A. sobria could cause obvious pathological changes in several tissues and organs of T. siluroides, such as serious pathogenicity of congestion, bleeding, degeneration, necrosis and inflammatory cell infiltration, especially in liver, kidney, spleen and intestines.

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