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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->1996 Vol.15 No.1

Studies on the DNA Fingerprinting in Two Species of the Gunes Triplophysa from China with Description of a New Species (Cyprinifomes: Cobitidae)
Author of the article:DING Rui-hua1, FANG Sheng-guo2, FANG Jing3
Author's Workplace:(1.Sichuan Institute of Natural Resource, Chengdu 610015; 2.Testing Center, Sichuan Normal university, 610066; 3. College of Animal Science and Technology, Sichuan Agricultural University, Yaan, Sichuan Province 625014)
Key Words:Cypriniformes; Cobitidae; Triplophysa; DNA fingerprinting; new species
Abstract: 

By combining DNA fingerprinting with classical taxonomic methods, this paper analysed the morphological characteristics and genetic relationship of two species of plateau cobitinae fishes (T. bleekeri and T. polyfasciata sp. nov.). The result shows that there might be a new species T. polyfasciata sp. nov. in T. bleekeri. Their outer morphology are so similar that they were negligently considered as one species T. bleekeri. By DNA fingerprinting, it has been found that their genetic characteristics are singficantly different. Every random sample of T. bleekeri has a similar hybridization band located at 3.0 Kb, while T. polyfasciata sp. nov. has an indentical band at position 6.0 Kb (Fig.2). The mean number of bands and the frenqency of differentiation of the former are 14.55±0.68 and 1.13 × 10-12, respectively, those of the latter are 21.76±1.08 and 7.65 × 10-21 (Tab.1). In appearance the former differs from the latter in the dorsal fin shorter, the position of dorsal fin slightly depressed, no stripe on the lateral body, while the latter has wide transverse stripe connected with dorsal one. Since there are great difference mentioned above in these two species, we consider that T. Polyfasciata sp. nov. should be separated as an independent species from T. bleekeri (Fig. 1, B)

The principal characteristics and diagnosis of the new species are given below.

Triplophysa polyfasciata Ding sp. nov. (Fig. 1.A)

Holotype: No.910093, male, total length 120 mm, body length100 mm, collected fromSanjiangVillage,WenchuanCounty. N,31, E, 103, altitude1200 m, Sichuan Province, a stream of upper Minjiang river, in 20 August 1991.

Allotype: No.9100137, female, total length108 mm, body length84 mm, collected with the hologype.

Paratype: 86 specimens, No. 910095-180, total length 98-160 mm, body length 80-137 mm, collected with the holotype. No. 9200146, collected from shuitanPingVillageTianquanCounty, altitude1290 m,UpperQingYijiangRiver.

Type specimens are in Sichuan Institute of Natural resource,Chengdu,Sichuan,China.

D. 8; A.- 5; P.- 11-12; V.- 7-8; C. 15-16-(branched), Gill rakers (inner) 10-13. Vertebrae (5 specimens) 4 + 34-35 + 1.

Depth of body 5.0-5.6(5.2±0.3), head length 4.0-5.0(4.7±0.5), length of peduncle 4.7-5.7(5.0±0.4) in standard length. Snout 2.0-2.5(2.3±0.2), eye 4.5-5.2(4.9±0.3), intherorbital space 3.5-4.1(3.2±0.3) in head length. Depth of caudal peduncle 1.6-1.9(1.8±0.1) in its length.

Body moderately elongate and cylindrical anteriorly. Without any scales, lateral line complete. Lips moderately thick. The male with a raised tuberculate area below the nares, and with thickened tuberculate pads on the back of the pectoral fin rays, lower lip is incised in the middle.

In appearance this new species appears to be similar to T. bleekeri (S. et D. 1874), but differs from the latter by following aspects: 1). the behind caudal fin dented deeply 2).the end of dorsal fin over the beginning of anal fin 3).in coloration, sides of body with 10-12 irregular dark brown cross bands in new species, but with distinct and irregular marking or blotches in the later T. bleekeri.
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