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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2013 Vol.32 No.4

Influence of Leptin to Somatic Cell Nuclear Transfer in Shanbei White Cashmere Goat
Author of the article:YU Honghao1,2, YAN Hailong1,2, ZHOU Li1,2, JING Xiaoqi1,2, HU Junhe1,2, FENG Ping1,2, QU Lei1,2*
Author's Workplace:(1. Life Science Research Center, Yulin University, Yulin, Shaanxi Province 719000, China;2. Shaanxi Cashmere Goat Engineering and Technology Center, Yulin, Shaanxi Province 719000, China)
Key Words:Leptin; nuclear transfer; Shanbei white cashmere goat; oocyte
Abstract:

The roles of Leptin are very complex. One of the important roles is regulation of energy metabolism. Some investigations showed that Leptin also had crucial role during the oocyte maturation and embryo development. Basic oocyte maturation medium with different concentrations of Leptin (0, group ; 10 ng/mL, group and 50 ng/mL, group) were used to mature Shanbei white cashmere goat oocytes. Skin fibroblasts from Shanbei white cashmere goat were used as donor cells, and enucleated matured oocytes from three groups were used as recipient ooplasm. Donor cells were introduced into perivitelline space of recipient ooplasm by microinjection, and the somatic cell cloned embryos were constructed after electric fusionSubsequently, the oocyte maturation rate in vitro, efficiency of somatic cell nuclear transfer and blastocyst rate of cloned embryos were analyzed to investigate the role of Leptin in nuclear transfer of goat. The results showed that the percentage of oocyte maturation in vitro and efficiency of somatic cell nuclear transfer were significantly higher in group  than the other two groups (P<0.05), and the percentage of blastocyst were no statistic different among the three groups (P>0.05). These results indicated that Leptin reduced maturation rate of goat oocyte in vitro and the efficiency of somatic cell nuclear transfer. However, Leptin had no effect on the developmental ability of cloned embryo. 

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