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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2013 Vol.32 No.4

Targeted Therapy of Expression Vector Containing AFP Regulation Sequence in Hepatoma Model
Author of the article:ZHANG Huanling, HOU Jie, WANG Junxia, YOU Hongyu, ZHENG Long, LIAN Weiguang, LIU Shufeng*
Author's Workplace:(Laboratory Animal Department of Hebei Medical University, Hebei Key Lab of Laboratory Animal, Shijiazhuang 050017, China)
Key Words:AFP promoter; expression regulation; P53; cell cycle; cell apoptosis
Abstract:Objective  To observe the targeted therapy of pAFP-P53-EGFP containing AFP regulation sequence in AFP positive hepatoma model. Methods  HepG2(AFP+), SMMC7721(AFP-) were inoculate in BALB/c-nu nude mice right axillary subcutaneous. AFP was detected by immunohistochemical. Tumor would be formed at 14 d. The pAFP –EGFP and pAFP-P53-EGFP recombinant plasmid were intratumorally injected and the tumor volume was monitored. The specific expression of p53 and cytotoxicity to HepG2 tumor was observed by immunohistochemistry. Results  The subcutaneous tumor of SMMC7721 and HepG2 cells were grew well, and the tumor volume was about 500 mm3. The successfully constructed model was confirmed by HE staining. AFP immunohistochemical results showed that AFP expressed in the HepG2 cells inoculated tumor tissue rather than SMMC7721. After treatment, p53 immunohistochemical analysis showed that expression of p53 in HepG2 cells inoculated nude mice treated with pAFP-P53-EGFP was significantly higher than other groups, and performed obvious apoptosis. The tumor volume was also smaller than the control group. Conclusion  The pAFP-P53-EGFP vectors containing the AFP gene regulatory sequences, which is capable of inducing cell cycle arrest and apoptosis, can be specifically applied to AFP positive hepatocellular carcinoma cells.
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