Comparison of Microsatellites between the Genomes of Tetranychus urticae and Ixodes scapularis
Author of the article:WANG Zili#, HUANG Jie#, DU Lianming, LI Wujiao, YUE Bisong, ZHANG Xiuyue*
Author's Workplace：(Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Science, Sichuan University, Chengdu 610064, China)
Key Words： Tetranychus urticae; Ixodes scapularis; genome; microsatellite; characterization
Abstract：The sequences of microsatellites in Tetranychus urticae and Ixodes scapularis genomes were compared in this study. A total of 7934 microsatellites were observed in T. urticae genome. The frequency was 1/11.45 kb and covering about 0.16% of the genome. Trinucleotide repeat motifs (72.83%) appeared to be the most abundant type in T. urticae genome. However, 550 629 microsatellites were observed in I. scapularis genome. The frequency was 1/3.21 kb and covering about 0.57% of the genome. The mononucleotide microsatellite was the most abundant type in I. scapularis genome and accounted for 73.74% of the total. The microsatellites in I. scapularis genome had more repeats than in T. urticae. What’s more, the microsatellites in T. urticae genome had a higher GC content (34.10%) than in I. scapularis (24.35%). The genome of T. urticae contained a higher proportion of unique microsatellite sequences than I. scapularis. Repeats of A, T, AG, TC, TG, GAT, ATTT and AATA were the common core sequences in the two genomes.