Sequence Analysis of Histone Deacetylase Genes in Mouse
Author of the article:WU Fengrui1,2, DING Biao1,2, LIU Yong1,2, LI Wenyong1,2*
Author's Workplace：(1. School of Life Science, Fuyang Teachers College, Fuyang, Anhui Province 236041, China; 2. Key Laboratory of Embryo Development and Reproductive Regulation in Anhui Province, Fuyang, Anhui Province 236041, China)
Key Words：histone deacetylases; gene; sequence analysis; mouse
Abstract：Based on NCBI and other bioinformatics database and software such as DNAstar and Clustal X, the gene structure, GC contents, open reading frame, amino acid sequence homology and chromosomal localization of 11 histone deacetylase (HDAC) genes of murine were analyzed. The results showed that the numbers of murine HDACs exons were between 10 and 29, the lengths were between 1044 bp and 3450 bp, and the GC contents were about 50%. Protein sequences analysis showed that HDAC 1 was highly homologous to HADC 2 (89.8%), however, HDAC 7 and HDAC 8 had only 8.8% homology. Furthermore, the 11 murine HDACs were separated into 4 different clades by the phylogenetic tree. Chromosomal localization analysis indicated that HDAC 6 and HDAC 8 were located in chromosome X, while the others were in euchromosome. In conclusion, these results revealed the basic biological characteristics of murine HDACs, and provided basic data for the research on the molecular mechanism and protein function of transcriptional regulation.