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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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PEI enhances in situ gene delivery and expression in skeletal muscles of BALB/c mice in the Pluronic-mediated system
Author of the article:Yutong He, Zhe Sun, Gang Wang
Author's Workplace:Engineering Research Center in Biomaterials, Sichuan University
Key Words:BALB/c mice, skeletal muscle gene delivery, plasmid, PEI, Pluronic L64
Abstract:Objective Cationic PEI is correctly introduced into Pluronic L64-mediated gene delivery system of mice skeletal muscle to enhance the efficiency of gene expression. Methods Different samples were transferred into mice tibialis anterior muscles via intramuscular injection in this experiment, among the naked plasmid DNA(pDNAs) and Pluronic L64/pDNA(L/D) were treated as the control groups, the mixtures of PEI/pDNA with different ratios and Pluronic L64 were treated as the experimental groups (L/P/D-0.5, L/P/D-3, L/P/D-10). Seven days after injection, these tissues were taken out, the gene expression and the potential pathological changes of muscle tissue were detected. In addition, the in vivo acute toxicity experiment was further executed via tail vein injection of L/P/D-0.5 group. Finally, the particle size, zeta potential and morphology of PEI/pDNA complexes in L/P/D-0.5 system were detected by DLS measurement and Atomic Force Microscope (AFM). Results ① The evaluation of report gene expression: the L/P/D-0.5 showed the highest gene expression; as N/P ratios increased, the gene expression could hardly be detected. ② The assessment of biocompatibility: no tissue lesion was observed in L/P/D-0.5 groups, regardless of muscular injection or intravenous injection. While other proportions of L/P/D showed histologic lesions with varying degrees. ③ Characterization analysis: PEI/pDNA complexes were spherical shape with nanometer size, which could contribute to gene delivery in skeletal muscle-based system, although pDNA was not fully compressed in L/P/D-0.5 group. Conclusion The L/P/D-0.5 system can safely and effectively enhance the expression of exogenous genes in skeletal muscle. Keywords: BALB/c mice, skeletal muscle gene delivery, plasmid, PEI, Pluronic L64
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