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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Application of DNA Barcoding in identification of encyrtid parasutoids of Acanthococcus lagerstroemiae
Author of the article:ZHANG Zeyuan, QIN Yaoguang, LI Shuqiang
Author's Workplace:1 Division of the High School Affiliated to Renmin University of China, Beijing 100080, China; 2 Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Key Words:Encyrtid parasitoids; Mitochondrial COI gene; Species identification; Acanthococcus lagerstroemiae
Abstract:Acanthococcus (=Eriococcus) lagerstroemiae (Kuwana) is an important pest of Punica granatum L. and Lagerstroemia indica L. and broadly distributed in China. Encyrtid parasitoids play a key role in the control of the A. lagerstroemiae. We obtained samples of the encyrtid parasitoids by rearing them from nymphs of Acanthococcus lagerstroemiae collected in different area of China. A DNA barcoding approach was applied to the identification of the encyrtid parasitoids following a preliminary morphological identification. DNA sequence data of the mitochondrial COI gene were generated from 93 individuals of the encyrtid parasitoids. The results show the inter-specific COI divergence was 0.0732~0.1864, averaged 0.1110; and the intra-specific COI variation was 0 ~0.0265, averaged 0.0074. The inter-specific COI divergence was greatly larger than the intra-specific COI variation for the encyrtid parasitoid species. Our results indicate, as a complementary approach to morphology, DNA barcoding can help us in rapid and accurate identification of the encyrtid species.
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