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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Molecular Cloning and Tissue Expression of Chicken GPR85 Gene
Author of the article:SONG Liang, ZHANG Jiannan, LIU Haikun, MO Chunheng, LI Juan, WANG Yajun
Author's Workplace:Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University
Key Words:chicken; GPR85; G protein-coupled receptors; clone; tissue expression
Abstract:G protein-coupled receptor (GPCR) is a kind of membrane receptor superfamily. It is an important receptor on cell transmembrane to transmit signals, which regulate many important physiological processes. Since the endogenous ligands for some GPCRs have not been identified, thus, these GPCRs have still been classified as orphan receptor. G protein coupled receptor 85 (GPR85) is a member of orphan receptor in GPCR superfamily. Till present, the information regarding on the structure and expression of GPR85 in non-mammalian vertebrates is limited. In this study, using RT-PCR and RACE PCR, we cloned the full cDNA sequence of GPR85 from chicken brain and revealed its gene structure. Furthermore, using quantitative real-time PCR, we also examined the mRNA expression of chicken GPR85 in various chicken tissues. The results showed that chicken GPR85 is located on chromosome 1 and consists of 2 exons. The coding region is located on exon 2. Chicken GPR85 has an open reading frame of 1113bp and encodes a seven-transmembrane receptor with a length of 370 amino acids. Chicken GPR85 shows extremely high amino acid sequence identity (>93%) with that of humans, rats, mice, western clawed frogs and zebrafish. qPCR revealed that chicken GPR85 is highly expressed in the brain regions, anterior pituitary, adrenal glands and testis, and weakly expressed in other remaining tissues examined. To our knowledge, this study represents the first to report the gene structure and expression of GPR85 in chickens and laid the foundation for further exploring the physiological roles of GPR85 in chickens.
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