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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Genetic variation of mitochondrial DNA D-loop region in different generations of wild and cultivated populations of Macaca thibetana
Author of the article:NIU Lihong
Author's Workplace:Institute of Laboratory Animal Sciences, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital
Key Words:Macaca thibetana; generations; mtDNA; D-loop; genetic variation
Abstract:To evaluate the genetic variation of offspring derived from wild and cultivated populations of Macaca thibetana, we analyzed the whole sequence of mitochondrial DNA D-loop region in the 102 individuals of two generations of wild (Jinyang region) and cultivated populations (Mabian region). The whole length of the region sequence was 1091 bp; 115 variable sites were detected. The 102 individuals presented 66 haplotypes, the two generations of Mabian and Jinyang population shared two and one haplotype respectively. The nucleotide polymorphisms (Pi) of two generations of Mabian population was 0.00477 and 0.00409 respectively while that in Jinyang population was 0.00230 and 0.00278, respectively, indicating no significant difference in genetic diversity occured between the offsprings and parents of M. thibetana. Genetic differentiation between the two generations of Mabian and Jinyang populations was 0.02409 and 0.00255 respectively, showing no significant differentiation happened in the two generations no matter in wild or domestic situation. NJ tree based on the whole length of D-loop showed two independent clades existed in Mabian and Jinyang populations while the sequence of different generations crossed each other in the NJ tree, demonstrating neither the random mating nor the artificial selection pressure exerted a significant influence on the genetic structure of the progeny. This study was the first time to use the whole length of mitochondrial DNA D-loop region to analysis the genetic diversity of wild and cultivated populations and different generations of Macaca thibetana. Our date provide date support for the standardization of genetic control of experimental animals.
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