刊期:双月刊
主管单位:四川省科学技术协会
主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
地址:四川省成都市武侯区望江路29号四川大学生命科学学院内
邮编:610065
电话:028-85410485; 15881112385
传真:028-85410485
E-Mail:scdwzz@vip.163.com & scdwzz001@163.com
刊号:ISSN 1000-7083
        CN 51-1193/Q
国内发行代号:
国际发行代号:
发行范围:国内外公开发布
定价:50元/册
定价:300元/年

您所在位置:首页->过刊浏览->2012年第31卷第3期

VX2肿瘤侵犯兔下腔静脉的生物学行为观察
Observation of biological behavior in rabbits of the VX2 tumor invading infrarenal IVC
张元国1,任为1*,张彦2,孙婧3
点击:913次 下载:1次
DOI:
作者单位:(1. 重庆医科大学附属第一医院血管外科,重庆 400016;2. 重庆医科大学检验医学院,重庆 400016;3. 重庆医科大学附属第一医院超声科,重庆 400016)
中文关键字:下腔静脉;VX2瘤;兔
英文关键字:inferior vena cava;VX2 tumor; rabbit
中文摘要:目的  建立VX2肿瘤侵犯新西兰兔下腔静脉(IVC)的动物模型,并观察相应的生物学行为。方法  经腹直视下,将VX2肿瘤组织块分别种植在28只兔肾以下IVC附近的软组织内,然后随机分为AB两组。A组术后每周在超声下观察肿瘤的大小、IVC和腹主动脉(AA)管径大小;DSA观察侧支循环建立。B组每周随机解剖4只,取IVCAA做病理和免疫组化(PCNA)检查,并观察转移情况。结果  1. 超声显示:所有肿瘤均种植成功,肿瘤面积大小与生长时间呈明显正相关(r=0.879)IVC管径与肿瘤面积大小显著负相关(r=-0.8295)4周时25%IVC管腔闭塞,5周时达到91.7%2. 病理和免疫组化显示:3周时IVC管壁中见大量肿瘤细胞;5周时AA管壁中未见肿瘤细胞。3. DSA显示:IVC慢性受侵犯过程中侧枝循环建立良好。4. 解剖观察:模型动物在第4周开始出现肝、肺和腹腔转移。结论VX2肿瘤成瘤率高,其生长变化、对IVC的侵犯和远处器官转移均随生长时间而变化明显。
英文摘要:Objective  To establish an experiment model of the VX2 tumorinvading IVC and observe its biological behavior. Methods  Abdominal nasopharynx, the prepared VX2 tumor tissue mass was transplanted into the soft tissues around the infrarenal IVC in 28 rabbits, then the rabbits were randomly divided into group A and group B. Group AThe size of tumor, the diameter of the IVC and the AA were detected by ultrasound once a week after 5 weeks implanted. The collateral circulation was observedwith DSA.Group B: 4 rabbits were randomly dissected weekly, the IVC and the AAwere sent to uptake pathologyand immunohistochemical analysis, and metastasis in lung, liver, kidney and celiac was observed at the same time. Results  1. Ultrasound examination shows: all VX2 tumors grow successfully in the model rabbits, the size of tumors were significantly positive related to time (r=-0.879, P<0.001), the diameter of IVC were significantly negative related to size of tumors(r=0.829, P<0.001).There were 25% lumen of IVC occluded in the first four weeks, and 91.7% in the first five weeks. 2. The pathological and immunohistochemical examination shows: a great quantity tumor cells were found in the wall of IVC and there was no tumor cell in the wall of the AA. 3. DSA examination shows: the collateral circulation developed well when the IVC was gradually invaded. 4. Anatomic observation: the tumor metastasized to lung and other organs in 3 weeks after implanted. Conclusion  Our study shows that tumor size, the diameter of the IVC and distant metastasis were significantly variable with time.
读者评论

      读者ID: 密码:   
我要评论:
国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
主管单位:四川省科学技术协会  主办单位:四川省动物学会/成都大熊猫繁育研究基金会/四川省野生动植物保护协会/四川大学
开户银行:中国工商银行四川分行营业部东大支行(工行成都东大支行营业室)  帐户名:四川省动物学会  帐号:4402 2980 0900 0012 596
版权所有©2019四川动物》编辑部 蜀ICP备08107403号-3
您是本站第7673769名访问者

川公网安备 51010702000173号