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多巴胺受体1在皖西白鹅小脑皮质发育中的表达
The Expression of DRD1 in the Development Cerebellar Cortex of Wanxi White Goose
宋海燕,刘再群*,郑磊
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作者单位:(安徽师范大学生命科学学院,安徽省重要生物资源保护与利用重点实验室,安徽芜湖241000)
中文关键字:皖西白鹅;小脑皮质;发育;DRD1;免疫组织化学
英文关键字:Wanxi white goose; cerebellum; development; DRD1; immunohistochemistry
中文摘要:采用普通染色及免疫组化SABC染色法研究皖西白小脑皮质的发育和多巴胺受体1(DRD1)阳性细胞在其发育中的表达。结果表明,小脑皮质在胚龄13 d (E13)由外向内分为外颗粒层(EGL)、浦肯野细胞层(PCL)和内颗粒层(IGL)E19由外向内分为EGL、分子层(ML)PCLIGL。随发育天数的增加,EGL的厚度和细胞层次呈先升后降的变化趋势,细胞密度逐渐下降;ML厚度逐渐增大,在E24E28时增值最大;浦肯野细胞(PC)E13E19E24E28随胚龄增大逐渐增大,在E28后趋于稳定,细胞密度随着发育天数的增加逐渐下降,在小脑皮质发育中还发现有一部分PC呈多层排列,且细胞层次逐渐变少;IGL厚度呈先升后降的变化趋势,细胞密度呈上升趋势。外颗粒层和内颗粒层在E13E19E24E28时有DRD1阳性细胞表达,分子层在E24E28、日龄7 d(P7)15 d (P15)有阳性细胞表达,PC在所检测的6个时段均有阳性表达。研究表明,小脑皮质的发育主要与细胞增殖、迁移和凋亡有关,外颗粒层的逐渐消失是以细胞迁移和凋亡为主,多层PC逐渐退化成单层是与细胞凋亡和正常突触联系的建立有关;DRD1在皖西白鹅小脑皮质发育中对外颗粒层细胞和PC起着重要作用。
英文摘要:The Nissl staining, HE staining or Holmes silver staining and strept avidin-biotin-peroxidase complex (SABC) immunohistochemical methodswere applied to investigate the development of cerebellar cortex in Wanxi white gooseand the expression ofDRD1 positive cells. Results show as follows: the cerebellar cortex at embryonic day 13 (E13) was consist of external granular layer, Purkinje cell layer and internal granular layer from outside to inside, at E19, which consists of external granular layer, molecular layer, Purkinje cell layer and internal granular layer from outside to inside. With the development day increases, the thickness and cell layers of external granular layer were both showed and then decreased, the cell density was declined; the thickness of molecular layerwas rising and maximized from E24 to E28; the Purkinje cell increased gradually from E13 to E28 and tends basically to steady after E28, the cell density of Purkinje cell was declined, experiments discovered that a part of Purkinje cells were arranged layers and the cell layers decreased gradually in the development of cerebellar cortex;the thickness of internal granular layer firstly increased and then decreased, the cell density was rising. In external granular layer and internal granular layer, there were DRD1 positive cells at E13, E19, E24 and E28; in molecular layer ,there were DRD1 positive cells at E24, E28, postnatal day 7 (P7) and P15; in Purkinje cell layer, there were DRD1 positive cells in 6 detected time points.Studies show that the development of cerebellar cortex was mainly related to cells proliferation, migration and apoptosis; the external granular layer disappears gradually because of the cells proliferation and apoptosis; that multi-layer Purkinje cells gradually degenerated into single layer Purkinje cells was related to cells apoptosis and the established of synaptic contact; DRD1 plays an important role in the cells of external granular layer and Purkinje cells in the development cerebellar cortex of Wanxi white goose.
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国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
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