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C57BL/6J-HBV转基因小鼠随增龄乙肝表面抗原表达及转氨酶水平变化探讨
Study on Changes in HBsAg Expression and Glutamic-pyruvic Transaminase Levels with Age in C57BL/6J-HBV Transgenic Mice
陈迪,田枫*,康爱君,王学文,郑振辉
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作者单位:(北京大学医学部实验动物科学部,北京 100191)
中文关键字:乙肝转基因小鼠;表面抗原;转氨酶
英文关键字:HBV transgenic mice; hepatitis B surface antigen; Glutamic-pyruvic transaminase
中文摘要:目的 探讨C57BL/6J-HBV乙肝转基因小鼠随增龄表面抗原表达及转氨酶水平的变化。方法 经血清表面抗原检测,根据S/C.O值将乙肝转基因小鼠分为两组,两组小鼠在4~10周龄每隔一周以酶联免疫吸附法测定血清表面抗原水平;各组小鼠在5~11周龄每隔一周以全自动生化分析仪测定小鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST)水平。结果 实验1HBV乙肝转基因小鼠表面抗原表达6周龄与4周龄相比显著增高,6周龄至10周龄趋于稳定;实验2组的雄性小鼠表面抗原表达6周龄与4周龄相比极显著降低(P0.01),而雌性小鼠在4周龄至10周龄表面抗原表达相对稳定。实验1组的雄性小鼠ALT 水平9周龄与7周龄比较显著下降(P<0.05),其余小鼠在5周龄至11周龄呈现下降趋势,但无显著性差异。实验1组的雄性小鼠AST水平11周龄与7周龄相比显著升高(P<0.05)结论 乙肝表面抗原S/C.O值不同的转基因小鼠表面抗原表达随增龄变化趋势不同;血清谷丙转氨酶和谷草转氨酶水平在5~11周龄变化趋势相反。
英文摘要:Objective To investigate the changes in HBsAg expression and glutamic-pyruvic transaminase levels with age in C57BL/6J-HBV transgenic mice. Methods HBV transgenic mice were divided into two groups according toS/C.O value. Serum HBsAg were measured by using ELISA assay every other week from four-weeks to ten-weeks in all mice and the glutamic-pyruvic transaminase level was determined by automatic biochemical analyzer every other week from five-weeks to eleven-weeks old. Results HBsAg expression in six-week old mice went up markedly compared with four-week old individuals in the first group.HBsAg expression in six-week old male mice went down significantly (P0.01) in comparison to four-week olds in the second group while no obvious changes were observed from four-weeks to ten-weeks old in female mice of the same group. ALT levels of nine-week old male mice was lower (P<0.05) than that of seven-week olds in the first group. AST levels of eleven-week old male mice was higher than that of seven-week old mice in the first group. Conclusion The change in HBsAg expression was different because of different S/C.O values and variations in ALT and AST was reversefrom five-week to eleven-week olds in HBV transgenic mice.
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国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
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